What Are the Different Types of Derivatives Jobs?

Derivatization is the use of chemical transformations to convert compounds into substances with similar chemical structures.

Derivatization is the use of chemical transformations to convert compounds into substances with similar chemical structures. In general, a specific functional compound participates in the derivatization reaction, solubility, boiling point, melting point,

The reactions commonly used for partial derivatization are esterification,

(1) Determination of chloramphenicol residues by gas chromatography with pre-column derivatization: After the sample is extracted, concentrated and purified, and dried with micronitrogen, 100 L of silylation reagent is added and reacted at 60 ° C for 30 min. The boiling point of chloramphenicol has been reduced and it can be used for gas chromatography or GC / MS analysis. DB-35MS (30m × 0.25mm × 0.25 & micro; m) column was used.

(2) Column derivation after lead oxide-HPLC method for determination of malachite green residues: malachite green (MG) belongs to triphenylmethane dyes. The colorless maternal form (leuco malachite green, LMG) is the main metabolite and existing form of malachite green in fish and other organisms. To detect the residue of malachite green in fish, MG and LMG should be detected at the same time. A C18 column (250 × 4.6mm ID, 5m) and a Celite 545 lead oxide column (pure PbO2 (1: 1), 20mm × 2.1mm ID) were used. Results When MG and LMG were detected simultaneously with crystal violet (GV) and the parent crystal violet, the retention times were: MG 8.33min, GV 14.18min, LMG 18.19min, and 19.84min. The use of lead oxide post-column derivatization-HPLC method for the determination of malachite green residues has the advantages of high separation efficiency, fastness, good reproducibility, and simple equipment.