What Is an Antibody Titer?

The antibody titer is used to measure the minimum concentration (ie, the maximum dilution) required for an antibody to recognize a specific epitope. Generally expressed as: the maximum dilution that still yields a positive result. Antibody titers are usually measured by ELISA methods.

Antibody titer

Right!

The antibody titer is used to measure the minimum concentration (ie, the maximum dilution) required for an antibody to recognize a specific epitope. Generally expressed as: the maximum dilution that still yields a positive result. Antibodies are usually detected by ELISA

For example, an indirect Coombs test is used to detect anti-Rh antibodies in the serum of a pregnant woman. A patient may report an antibody titer of 16 for this test, which means that as long as the serum

Source: Chinese Poison

Method used

The detection method is the same as above [11] .A450

Source article abstract: Gene fragments encoding the three antigens of Mycobacterium tuberculosis Ag85B, MPT64, and MPT83 were inserted into eukaryotic expression vectors as a combined vaccine to immunize mice, and DDA and MPL as adjuvants respectively improved the immunogenicity and immune protection of this trivalent vaccine Effect, and DDA is better than MPL. After adding DDA, Ag85B, MPT64, and MPT83 antigen-specific IFN- contents were (265.37 ± 79.2) U / ml, (185.31 ± 58.3) U / ml, (108.13 ± 54.4) U / ml, which is 16 U / ml, 45 U / ml, and 2 U / ml higher than that of the non-adjuvant group, respectively, and there is no significant difference with the content of three antigen-specific IFN- in the MPL group. There was no significant difference in each group. The results of bacterial counts after challenge showed that the lung and spleen load of the trivalent vaccine group with adjuvants was reduced by 2 to 3 orders of magnitude compared with the empty vector group, and the adjuvant DDA The group was significantly better than the adjuvant MPL group and the non-adjuvant group. The pathological section results were consistent with the bacterial load data. The adjuvant group, especially the DDA group, had relatively reduced lung lymphocytes and increased macrophages. DDA as an adjuvant can significantly improve the immune efficiency of nucleic acid vaccines.Adjuvant MPL cannot improve the immune efficiency of multivalent nucleic acid vaccines of Mycobacterium tuberculosis.