What Is Osteolysis?

The lysis technique is to mix spleen cells isolated in a sterile state with myeloma cells in a logarithmic growth phase at a certain ratio. A successful fusion depends on prior immunization and reliable screening after fusion.

Lysis

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For lysis, spleen cells isolated in a sterile state and myeloma cells in logarithmic growth phase are mixed in a certain ratio.

The lysis technique is to mix spleen cells isolated in a sterile state with myeloma cells in a logarithmic growth phase at a certain ratio. A successful fusion depends on prior immunization and reliable screening after fusion.

Ma Lixian and Shao Lihua pointed out in "Study on the Fusion Effect of Human Embryonic Kidney Cells Caused by Renal Syndrome Hemorrhagic Fever Virus": In order to explore the relationship between renal syndrome hemorrhagic fever virus (HFRSV) and human cell fusion, 4 strains of HFRSV (76 118, A9, R22, L99) infected human embryonic kidney cells. After IFA and IPA detection (++++), a cell test was performed at pH 4.5-8.0 and 37 ° C. The results showed that: 4 All strains of the virus can cause human embryonic kidney cell fusion, and the optimal pH is 5.0-6.5. This fusion phenomenon can be neutralized by the serum of patients with HFRS recovery period. Studies have confirmed that HFRSV can cause human embryonic cell fusion.