What Are the Most Common Methotrexate Side Effects?
Methotrexate, an antifolate antitumor drug. This product is orange-yellow crystalline powder. Mainly through inhibition of dihydrofolate reductase, the tumor cell synthesis is inhibited, and the growth and reproduction of tumor cells are inhibited.
Methotrexate
- Methotrexate, an antifolate antitumor drug. This product is orange-yellow crystalline powder. Mainly through
- [Main ingredients]: The main ingredient of this product is methotrexate.
- This product is in
- Method name: Methotrexate API-Methotrexate-High Performance Liquid Chromatography
- Scope of application: This method uses high performance liquid chromatography to determine the methotrexate content in methotrexate drug substances.
- This method is applicable to methotrexate APIs.
- Principle of the method: The test product was dissolved in the mobile phase and quantitatively diluted, and then chromatographed by high-performance liquid chromatography. The peak area of methotrexate was detected at a wavelength of 302 nm using a UV absorption detector, and its content was calculated.
- Reagent: 1. Acetonitrile
- 2. 7.0% citric acid solution
- 3. 2.0% anhydrous disodium hydrogen phosphate solution
- Equipment: 1. Instrument
- 1.1 HPLC
- 1.2 Column
- Octadecylsilane-bonded silica gel is used as a filler, and the number of theoretical plates should not be less than 1,000 calculated based on methotrexate peak.
- 1.3 UV absorption detector
- Chromatographic conditions
- 2.1 Mobile phase: acetonitrile 7.0% citric acid solution 2.0% anhydrous disodium hydrogen phosphate solution = 10 10 80
- 2.2 Detection wavelength: 302nm
- 2.3 Column temperature: room temperature
- Sample preparation: 1. Preparation of reference solution
- Accurately weigh an appropriate amount of the methotrexate reference substance, add the mobile phase to dissolve and quantitatively dilute it to a solution containing about 0.1 mg per 1 mL, which is the reference solution.
- 2. Preparation of test solution
- Accurately weigh the appropriate amount of the test product, add the mobile phase to dissolve and quantitatively dilute it to a solution containing about 0.1 mg of methotrexate per 1 mL, which is the test solution.
- Note: "Precision weighing" means that the weighed weight should be accurate to one thousandth of the weighed weight. "Precision measurement" means that the accuracy of measuring the volume should meet the accuracy requirements of the volume pipette in national standards.
- Operation steps: Precisely draw 10 mL of each of the reference solution and the test solution, inject them into a high-performance liquid chromatograph, and use a UV absorption detector to measure the peak area of methotrexate (C20H22N8O5) at a wavelength of 302 nm and calculate its content.
- References: Pharmacopoeia of the People's Republic of China, compiled by the National Pharmacopoeia Committee, Chemical Industry Press, 2005 edition, one, p.120.
- Tetrahydrofolate
- When the dosage is less than 30mg / m 2 , oral absorption is good, and the plasma concentration reaches the highest peak within 1 hour to 5 hours. Partial warp
- (1) Systemic medication
- Mainly suitable for
- 1 The treatment of leukemia is usually 2.5 mg to 10 mg per day for adults, with a total amount of about 50 mg to 150 mg. Children 1.5mg 5mg / day.
- 2 For treatment of chorionic epithelial cancer, such as 10mg-20mg / day, intramuscularly or orally, can also be given intravenously for 5-10 days,
- 1. Ethanol and other drugs that are harmful to the liver, if used with this product, it can increase liver toxicity;
- 2.Because it can cause blood
- Side effects:
- 1 Gastrointestinal reactions are mainly stomatitis, oral ulcers, pharyngitis, nausea, vomiting, gastritis and diarrhea.
- 2 Myelosuppression is mainly manifested as
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