What Is a Dihybrid Cross?

The two-hybrid system consists of a reporter gene transcriptional regulatory region, a reporter gene, and a pair of interacting trans-acting factors. The single hybrid system technology developed from the above hybrid system is also based on the detection of the expression level of the reporter gene to screen out the DNA of unknown factors combined with known cis-acting elements. Trans-acting factors that are weak and difficult to purify by biochemical means. ^[1]