What is endonuclease?

endonuclease is a specialized enzyme that is able to break the bonds between nucleic acids in the polynucleotide chain, providing two fragments. These enzymes can act in both DNA and RNA to reduce from the spring. Some endonuclease, known as restrictive endonuclease, attack specific places in the sources of RNA and DNA, recognize specific sequences. These enzymes are very valuable in scientific research when people want to adjust genetic information, as in genetic engineering. Endonuclease use bacteria and archaea as part of their defensive mechanisms. Some of the first observations of these enzymes in effect come from bacteria research and bacteria are used to produce endonucleases on a large scale for research purposes. The organism protects its own DNA from the action of endonuclease process known as methylation. Methylation prevents the behavior of endonuclease and ensures that the DNA of the organism is left intact. Foreign DNA, however, is not such a tankIt gets out and the endonuclease neatly cuts the backbone of the bottom in two places to separate it to form separate springs. Depending on which endonuclease is involved, the events of "dull" or "sticky" may be, referring to where it is interrupted in the DNA or RNA spring.

Scientists can use well -known restriction endonuclease to blast DNA in the desired target areas and create a convenient insert point. At this point, a new genetic material can be inserted to get an expression of a completely new gene. This can be used to do things like introducing resistance to herbicide to crops so that farmers can spray fields to keep down weeds.

Labs, which produce endonucleases, sell their products to scientists and other laboratory. The laboratory describes the ways the product can act, allowing scientists to determine which product would be best for their desired use. Work with endonUlleasis can be complex and requires patience and skill. You could think of an enzyme, such as restrictive endonuclease as a very small and very fine set of scissors that can be used to create a project, but can also fail if it is not properly managed, cut in the wrong place or not cut at all.