What Is a UV Detector?

Ultraviolet absorption detector is referred to as ultraviolet detector for short. It is a detector designed based on the principle that solute molecules absorb ultraviolet light. Its working principle is the Lambert-Beer law. Light, the absorbance A is proportional to the concentration C of the light absorbing component and the optical path length L of the flow cell.

Physically measured

Flow cell maintenance

For lightly polluted flow cells. Can be cleaned in-line with the flow cell. For heavily polluted flow cells, in-line flushing will generally not work. The flow cell assembly should be removed from the instrument for cleaning.

The procedure is as follows: Loosen the fixing screw located on the upper end of the sample flow cell. Take out the flow cell assembly and remove the joints at the entrance and exit respectively; then remove the three stainless steel screws after removing the three screws that fix the window, then you can remove the two quartz windows of the flow cell. Be careful not to lose the plastic king seal on the contact part of the window with the flow cell body. The windows and the flow cell body were respectively placed in 2 beakers filled with methanol and ultrasonically cleaned for 20 min. If the pollution is particularly serious, you can replace it

UV detectors are used in most common

The coverage of the UV detector reaches 75% of the HPLC detector, and it has been widely used in various fields, especially in the fields of pharmaceuticals, environmental protection, life sciences, food science, agricultural science, food science, medical and health, etc. . There are many international manufacturers producing HPLC, and all have UV detectors. There are also more than 10 companies producing HPLC in China. Basically all have UV detectors. Some HPLCs have only one UV detector, but no other type.