What Does an Agricultural Scientist Do?

Liu Baohui was born in Heilongjiang Province in July 1964. He graduated from Northeast Agricultural University with a bachelor's degree in 1987 and a doctor's degree in 2003. From 2001 to 2008, he was a special researcher and postdoctoral fellow in applied life sciences at Hokkaido University in Japan. He was selected into the "Hundred Talents Program" of the Chinese Academy of Sciences in 2008. He is currently a researcher and PhD supervisor of the soybean molecular breeding laboratory of the Northeast Institute of Geography and Agricultural Ecology of the Chinese Academy of Sciences.

Liu Baohui

(Agricultural scientist)

Genetic and Functional Genes of Soybean Important Traits

Main research contents: Using soybean as research material, clone functional genes (or QTLs) that control important traits and conduct in-depth biological function research in order to clarify the molecular regulatory mechanism of related traits and provide a theoretical basis for molecular breeding. Research involves genetics, bioinformatics, gene chips, genetic engineering, plant physiology, and histocytology.

1 . Mapping QTLs for 9 Important Soybean Traits

Using cultivated soybean (max) Tokei 780 and wild soybean (soja) Hidaka 4 as parents, a soybean permanent mapping population RIL (Recombinant inbred lines) was constructed. This group was used to locate the flowering time (photosensitivity), growth habit (Determinate habit), twining habit, maximum internode length, number of nodes, and plant height (Plant height), Pod dehiscence (Shattering), Hardness or Water absorption (Hard seededness), Seed weight (100-seed weight) of 9 important cultivation traits QTL. Use 282 There are 204 SSR markers, 5 isozyme markers, 73 AFLP markers, covering the genome of 2383CM, an average map distance of 8.5CM, a total of 17 QTLs were detected, and they were distributed on 10 linkage groups. Especially D1b and Hard QTLs and pod split QTLs in the J-linked group were also detected in other mapping groups. It was proposed that these common QTLs play an important role in the process of soybean cultivation.

2 First cloned E4 gene during soybean growth period (photoperiod sensitivity) and verified its function

Multiple homologous sequences of the light-sensitive pigment A gene were found in the soybean genome, and the DNA sequences of two homologs of the designated GmphyA1 and GmphyA2 genes were determined. Analysis of the GmphyA2 gene based on a homologous line carrying a recessive gene at the light-insensitive E4 site revealed that a retrotransposon was embedded in exon 1 of the gene, resulting in a gene disorder. The mapping reveals that GmphyA2 is encoded by E4. It is proposed that genetic excess means that the presence of a duplicate copy of the phyA gene is responsible for the insensitivity of the photoreaction and prevents the harmful effects of mutations; there is a linkage between gene replication and the ability of plants to adapt to the environment.

3 For the first time, we cloned the soybean pod habit Dt1 gene using a map-based cloning method and allowed it to be overexpressed in soybean and used RNAi technology to reduce its expression in soybean to verify its function

The Dt1 gene of soybean pod habit was cloned in the map, and BLAST analysis showed that Dt1 was an Arabidopsis TFL1 homologous gene. Analysis of near isogenic lines showed that dt1 is a mutation type in which a single base mutation occurs in the Dt1 coding region and a single amino acid is replaced. Excessive expression of TFL1b in soybean by transgenic technology and its reduced expression in soybean using RNAi technology have verified its function. A method was developed to identify soybean pod habit types.

1. Baohui Liu , Akira Kanazawa, Hisakazu Matsumura, Ryoji Takahashi, Kyuya Harada, Jun Abe. Genetic Redundancy in Soybean Photoresponses Associated With Duplication of the Phytochrome A Gene. Genetics, 2008 180 (2): 995-1007.

2. Baohui Liu , Fujita T, Yan ZH, Sakamoto S, Xu D, Abe J. QTL Mapping of Domestication-related Traits in Soybean (Glycine max). Ann Bot, 2007, 100: 1027 ~ 1038; doi: 10.1093 / aob / mcm149.

3. Baohui Liu , Ryoji Watanabe, Kyuya Harada, Jun Abe. Soybean TFL ortholog is associated with a determinate habit gene (dt1). Plant Journal. (In review)

The content of Baidu Encyclopedia is edited by netizens. If you find your entry is inaccurate or incomplete, you are welcome to use my entry editing service (free of charge) to participate in the correction. Go Now >>