What Are the Effects of High Thyroxine Levels?
Serum thyroid stimulating hormone (TSH), which is a hormone secreted by the pituitary gland, mainly functions to promote iodine uptake by the thyroid gland and accelerate thyroid hormone synthesis and secretion. Serum thyroid stimulating hormone is an important indicator for the diagnosis of primary hypothyroidism.
- Thyroid stimulating hormone
- 1. TSH reduction: seen in secondary hypothyroidism, diffuse goiter with hyperthyroidism and other symptoms.
- 2. Increased TSH: seen in
- 1. Direct Chemiluminescence Immunoassay (CLIA)
- Principle: The antibody (antigen) is directly labeled with acridinium ester, and after an immune reaction with the corresponding antigen (antibody) in the test sample, a solid-phase coated antibody-the test antigen-an acridinium ester-labeled antibody complex is formed. The oxidant (H 2 O 2 ) and NaOH are added to make it into a basic environment, and the acridine ester decomposes and emits light without the need of a catalyst. The luminous intensity (RLU) is measured, and the specimen content can be calculated from the calibration curve.
- 2. Chemiluminescence enzyme immunoassay (CLEIA)
- CLEIA uses an enzyme that catalyzes a chemiluminescence reaction, such as horseradish peroxidase (HRP) or alkaline phosphatase (ALP), to label an antigen or antibody, and immunizes with the corresponding antigen (antibody) in the test sample. After the reaction, a solid-phase coated antibody-antigen to be tested-enzyme-labeled antibody complex is formed. After washing, a substrate (luminescence agent) is added, the enzyme catalyzes and decomposes the substrate to emit light, and measures its luminous intensity (RLU). The content of the specimen can be calculated from the calibration curve.
- 3. Electrochemiluminescence immunoassay (ECLIA)
- The detection method is an electroluminescent agent called terpyridine ruthenium-labeled antibody (antigen), and tripropylamine (TPA) as an electron donor. A specific chemiluminescence reaction occurs due to electron transfer in an electric field. In the electrochemiluminescence immunoassay system, the magnetic particles are coated with an antibody (antigen) as a solid phase carrier, and the antibody (antigen) is labeled with a tripyridine ruthenium. In the reaction system, the sample to be tested reacts with the corresponding antigen (antibody). Then, a magnetic particle-coated antibody-antigen to be tested-terpyridine-ruthenium-labeled antibody complex is formed. At this time, the complex is sucked into the flow chamber, and a TPA buffer is introduced. When the magnetic particles flow through the electrode surface, it is installed on the surface of the electrode. The electromagnet under the electrode is attracted, and the unbound labeled antibody and specimen are washed away by the buffer solution. At the same time, the electrode is pressurized, and the electrochemical luminescence reaction is started, so that the electron transfer of terpyridine and TPA on the electrode surface generates electricity. Chemiluminescence, the intensity of the light is directly proportional to the concentration of the test antigen. [4]