What Are the Most Common Uses for Hydrocortisone Acetate?
Introduce
Hydrocortisone acetate
- Introduce
- [Chinese name] hydrocortisone acetate
- [Chinese alias] hydrocortisone-21-acetate; hydrocortisone acetate; ifosfamide; hydrocortisone acetate; hydrocortisone acetate
- [English name] Hydrocortisone Acetate
- [Other names] Hydrocortisone, Cortisone, Corsoline,
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- Method name: Hydrocortisone Acetate-Determination of Hydrocortisone Acetate-High Performance Liquid Chromatography
- Application: This method uses high performance liquid chromatography to determine the content of hydrocortisone acetate.
- This method is applicable to hydrocortisone acetate.
- Principle of the method: After the test sample is processed, it is dissolved in mobile phase and diluted to a certain concentration solution. It is then chromatographed by high performance liquid chromatography, and the absorption value of hydrocortisone acetate is detected at a wavelength of 254 nm using a UV absorption detector. And calculate its content.
- Reagents: 1. Acetonitrile (chromatographically pure)
- 2. Water (chromatographically pure)
- 3. Methanol
- Equipment: 1. Instrument
- 1.1 HPLC
- 1.2 Column
- Octadecylsilane-bonded silica gel is used as a filler. The theoretical number of plates calculated from the peak of hydrocortisone acetate is generally 6000. The resolution of hydrocortisone acetate and cortisone acetate should be greater than 5.5.
- 1.3 UV absorption detector
- Chromatographic conditions
- 2.1 Mobile phase: acetonitrile: water = 38 62
- 2.2 Detection wavelength: 254nm
- 2.3 Column temperature: room temperature
- Sample preparation: 1. Preparation of reference solution
- Take 11mg of hydrocortisone acetate, accurately weigh, place in a 50mL volumetric flask, add an appropriate amount of methanol, dissolve with ultrasound, let cool, dilute to the mark with methanol, shake well, take 2mL accurately, place in a 25mL volumetric flask, add Dilute the mobile phase to the mark and shake well.
- 2. Preparation of test solution
- Take an appropriate amount of this product, accurately weigh, add methanol to dissolve and quantitatively dilute to make a solution containing 0.22mg per 1mL, accurately measure 2mL, place in a 25mL volumetric flask, add mobile phase to dilute to the mark, and shake well.
- Operation steps: Precisely draw 20 L of each of the reference solution and the test solution into a high-performance liquid chromatograph, and use an ultraviolet absorption detector to measure the absorption value of hydrocortisone acetate at a wavelength of 254 nm, and calculate its content. [1]
- [Usage and dosage]
- 1. Eye drops: After shaking, drip into the conjunctival sac, 3 to 4 times a day, 1 to 2 drops at a time.
- 2. Eye ointment: Apply to the eyelids, a little at a time, 2 to 3 times a day, the last time should be used before bedtime.
- [Preparation and specifications]
- 1. Hydrocortisone acetate eye drops: 3ml: 15mg; 5ml: 25mg.
- 2. Hydrocortisone acetate eye ointment: 0.5%.
- For the rest, please refer to the Adrenocortical Hormone section of the Endocrine System Medication chapter.
- Application of corticosteroids in large doses for a long time can cause iatrogenic adrenocortical hyperfunction (Cushing-like syndrome), manifested as full moon face, buffalo back, centripetal obesity, thin and purple skin, and muscle atrophy. , Osteoporosis, hyperglycemia, diabetes, hypertension, hypokalemia, virilization of women such as hair, beard, amenorrhea, rough voice, etc. (metabolites have a weak androgenic effect), inhibition of child growth and development, fetal malformations, induction Infection spread, ulcer induced, psychotic induced, diabetes induced, hypertension induced, pancreatitis induced; withdrawal syndrome (myalgia, myotonia, arthralgia, etc.); rebound phenomenon (decreased too quickly or abruptly discontinued, Original symptoms may appear or worsen quickly); iatrogenic adrenal insufficiency (adrenal crisis): in long-term high-dose application, due to negative feedback, endogenous glucocorticoid secretion is reduced, and even adrenal cortex atrophy , After sudden withdrawal, if you experience stress, due to endogenous
- Hydrocortisone acetate
- Cusuan Qinghua Kedisong
- Hydrocortisone Acetate
- Page number: 2005 edition two-850
- [Revised]
- [Properties] "This product is slightly soluble in ethanol or chloroform and insoluble in water." Was changed to "This product is slightly soluble in methanol , ethanol or chloroform and insoluble in water."
- [Inspection] Take about 25mg of this product, put it in a 50ml measuring bottle, add 20ml of acetonitrile, dissolve by sonication, cool to room temperature, dilute with water to the mark, shake well, and use it as a test solution (used within 12 hours) ; Precisely measure 1ml, place it in a 100ml measuring flask, dilute to the mark with mobile phase, shake well, and use it as a control solution. According to the chromatographic conditions under the content determination item, take 20 l of the control solution and inject it into the liquid chromatograph, and adjust the detection sensitivity so that the peak height of the main component chromatographic peak is about 50% of the full scale. Then 20 l of each of the test solution and the control solution were precisely measured and injected into the liquid chromatograph respectively. The chromatogram of the test solution was recorded to 3 times the peak retention time of the main component. If there is an impurity peak in the chromatogram of the test solution, the maximum single impurity peak area should not be larger than the main peak area of the control solution (1.0%) , and the other single impurity peak area should not be larger than 1/2 (0.5%) of the main peak area of the control solution. The sum of the peak areas must not be greater than 2 times (2.0%) the main peak area of the control solution (any chromatographic peak in the test solution that is less than 0.02 times the main peak area of the control solution can be ignored).
- [Content determination] According to high performance liquid chromatography (Appendix VD).
- Chromatographic conditions and system suitability tests use octadecylsilane bonded silica as a filler; acetonitrile-water (36:64) as a mobile phase; detection wavelength is 254nm. Take the appropriate amount of hydrocortisone acetate and cortisone acetate reference, accurately weigh, dissolve with mobile phase and make a mixed solution containing 5g per 1ml , measure 20l into the liquid chromatograph, adjust the flow rate, make acetic acid The retention time of the hydrocortisone peak is about 16 minutes, and the resolution of the hydrocortisone acetate peak and the cortisone acetate peak should be greater than 5.5.
- Determine the appropriate amount of the product by measuring , accurately weigh, dissolve with methanol and quantitatively dilute to make a solution containing 0.25mg per 1ml; take 5ml for precise measurement, place it in a 25ml measuring flask, dilute to the mark with mobile phase, shake well, precision Measure 20l into the liquid chromatograph and record the chromatogram. Another appropriate amount of hydrocortisone acetate reference is accurately weighed and measured in the same way. Calculate the peak area according to the external standard method.
- [REDACTED]
- [Character] Melting point The melting point (Appendix VI C) of this product is 216 224 ° C. It will decompose at the same time when melting.