What Is Triptolide?

Triptolide, also known as triptolide, triptolide, is an epoxy diterpene lactone compound extracted from the roots, leaves, flowers and fruits of the plant Euonymus triptolide, and it is related to triptolide and triptolide Alkaloids such as vinegarine, tripterygium alkaloids, triptolide, tripterygium soda and tripterine alkaloids constitute the main active ingredient of tripterygium wilfordii extract. Sulfoxide, absolute ethanol, ethyl acetate, chloroform, etc. With anti-inflammatory and immunosuppressive effects.

Triptolide

Triptolide, also known as triptolide, triptolide, is derived from

CAS # 38748-32-2

Molecular formula: C20H24O6

Molecular weight: 360.4

Appearance: white needle crystal

Solubility: Melting point: 226 227 ° C. Insoluble in water, soluble in methanol, ethyl acetate, chloroform, etc.

Save: -20 oC

Purity: 95% ~ 99%
Analytical method: HPLC-DAD or / and HPLC-ELSD
Identification method: Mass spectrometry (Mass), Nuclear magnetic resonance (NMR)

(1) Chromatographic conditions: silica gel G-CMCNa plate; chloroform-ether (2: 1) as the developing solvent; 2% 3,5-dinitrobenzoic acid ethanol solution and 2mol / L sodium hydroxide ethanol solution. Immediately before use, mix 1: 3 as a color developer, spray color, and view under natural light.

(2) Preparation of reference solution: Weigh an appropriate amount of triptolide reference solution, add chloroform to make a solution containing 1.528 mg per milliliter, and use it as the reference solution.

(3) Preparation of sample solution: accurately weigh the appropriate amount of dried medicinal materials, add ethanol to reflux and extract 3 times for 3h each time, combine the extracts, recover the ethanol to dryness, dissolve the extract with ethyl acetate and extract, and inspect the thin layer of the extract No triptolide exists. Recover ethyl acetate to obtain the extract. Weigh precisely 0.4 g of extract powder dried at 90 ° C to constant weight, dissolve it in 5 ml of dichloromethane, mix with 8 g of neutral alumina, and distill. Chloromethane is transferred into a Soxhlet extractor and refluxed with petroleum ether (60-90 ° C) for 3 hours. The petroleum ether is discarded, the filter paper tube is removed, the solvent is evaporated, and then placed in the extractor and refluxed with 70 ml of chloroform for 5 hours. The chloroform is recovered to Dry, let cool, and add 3ml of chloroform precisely to dissolve it as a sample solution.

(4) Measurement: Precisely pipette 10 l of sample solution, 2, 7 l of reference solution, and point on the same thin plate. Develop according to the above chromatographic conditions, remove, dry, and develop color. Cover the glass plate of the same size, and seal it with adhesive tape for 4 weeks. After 10 minutes, scan by thin layer scanning method. S = 535nm, R = 650nm; dual-wavelength reflection method sawtooth scanning; slit: 1.25mm × 1.25mm; sensitivity × 5. Measure the integrated value of the absorbance of the sample and the reference substance, and calculate the content by the two-point method of external standard.

(5) Measurement results: According to the above conditions, the contents of triptolide in the root bark, root core and whole root of tripterygium wilfordii were respectively 0.001%, 0.0004%, and 0.0018%.

Chinese name Tripterygium

CAS NO. 38748-32-2

Chinese alias triptolide; triptolide A; triptolide; triptolide (triptolide) TLC; triptolide (triptolide)

English alias PG490; TRIPTOLIDE, TRIPTERYGIUM WILFORDII;, 7as, 7bs, 8as, 8bs)-; 8b, 9,10-decahydro-6-hydroxy-8b-methyl-6a- (1-methylethyl)-, (3bs, 4as, 5as, 6r, 6ar; TRIPTOLIDE TLC; TRIPTOLIDE95%; TRIPTOLIDE (P); TRIPTONIDE (P); (3bS, 4aS, 5aS, 6R, 6aR, 7aS, 7bS, 8aS, 8bS) -3b, 4,4a, 6, 6a, 7a, 7b, 8b, 9,10-decahydro-6-hydroxy-8b-methyl-6a- (1-methylethyl) -trisoxireno [4b, 5: 6,7: 8a, 9] phenanthro [1,2- c) furan-1 (3H) -one; NSC 163062