What is a gene resistance to Kanamycin?

Kanamycin resistance to resistance (NPTII or NPTIII) is a DNA chain that allows the body to produce protein, giving resistance to a common antibiotic of canamycin. This gene is often used as a selective marker for exogenous plasmids - plasmids that do not naturally occur - in organisms such as bacteria or yeast. This selection agent is also used in plants. Scientists who study genetics or proteomics can choose for bacterial colonies that include an embedded gene that is interesting based on the use of canamycin. Kanamycin kills every cell colony that does not include cells of transcription and translation of the gene with associated resistance. Any cell that can read this gene and rewrite the resulting enzyme will have resistance for kanamycin. This gene was isolated from a resistant bacterial strain and copied to other plasmids. Using enzymes, scientists can design plasmids that include resistors against selection agents such as Kanamycin.

There are many paths that resistance to aminoglycosides such as kanamycin. Genetic resistance to kanamycin may be the result of reduced cell permeability or cellular inactivation of the enzyme of canamcin. It is also possible that the cell shows resistance to canamin chromosomal change leading to a change in ribosomes of this cell. However, this latest resistance is not as useful for genetics as other ways, because it relies on chromosomal DNA and non -plasmid. In other words, this resistance is naturally occurring and cannot be inserted.

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gene resistance to Kanamycin has some crossover resistance to other antibiotics and selection substances such as gentamycin and neomycin. This feature causes a gene resistance to kanamycin is less useful because wide selection substances prevent a specific selection of bacterial strains. In other words, if a scientist wanted to study interactionor two plasmids, inserted by both into a unicellular organism, such as yeast, the scientist could not use the resistance to neomycin or gentamycin as a selection marker, if the canamycin already relies.

Kanamycin resistance is usually used in laboratories and has become a common selection agent for use in genetically modified organisms. As one of the most common antibiotics, it is assumed that Kanamycin exists in abundance. As a result, there are few restrictions on the use of canamin in plant transgenic and genetic modifications of plants for large industrial agricultural production.