What is a complicated human protein?

Recombinant human protein is a human protein that is produced from cloned DNA. This allows scientists to express its large quantities. Such excessive expression had great usefulness for modern medicine, allowing the production of drugs based on human proteins that have no other source. This also led to great progress in understanding the function and biology of human proteins. This hormone controls the production of red blood cells. It is used to treat anemia from various sources, including chronic kidney disease and cancer. Erythropoetin was also used as a medicine to improve the performance of athletes. An example is the human growth hormone, which is currently obtained for therapeutic use using recombinant techniques. The third method of insulation from the corpses has sometimes led to the transmission of diseases. Insulin is another medicine that is used as a remombinant human protein. Most of the insulin used by patients are obtained in this way.

Protein production from cloned genes is feasible as genes may be KLhe is heard into express vectors. These are specialized DNA units that are designed to produce a large amount of protein using specialized promoters. These promoters control the production of cloned gene sequence. Own sets are available for cloning and expression of proteins.

Specialized host cells are required to produce a technical human protein. They may be bacterial or yeast cells. Some proteins require special modifications such as the introduction of sugars, and are expressed in more advanced cellular lines such as mammalism or insect cell lines.

F or bacterial cells will be proteins inside the cells, requires that extraction and cleaning of the protein separate them from bacterial proteins. This is facilitated by special techniques that are part of the cloning process. For example, specialized binding areas may be cloned that allowProtein to bind to the matrix and easily eludes. This can save years of developing protein cleaning methods. Recombinant human proteins expressed in mammal cell lines are often excreted in the media, which facilitates their insulation and cleaning.

Have genes for proteins available as clones allow scientists to create their own proteins and change them to make the properties they want. For example, some suggestion insulin has been genetically changed to have different effects on the body. The ability to change these proteins is very useful in biological research.

The ability to express a complicated human protein revolutionized Biomedicial Research. When a scientist clone the gene, he can compare it with a huge database of known gene sequences. If the sequence gene, which is highly similar to the sequence of the gene of known function, can predict the function of this gene. This knowledge suggests which experiments perform with the product product, which is often a protein. Sometimes there is no homology with other gene sequencesAnd the scientist does not know the function of the gene.

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Expromanting gene product allows scientists to test the function of the gene using biochemical techniques. This can allow him to identify the function of the gene. It can also perform experiments with Messenger RNA (MRNA) produced directly from the gene and determine under what conditions and in which tissues the gene is expressed. This knowledge helps to narrow in searching for a gene function and see if it encodes protein.

If it is known to the function of protein, excessive expression can provide large amounts of protein to study its biochemical properties. It can make targeted mutations and see what effects they have on protein properties. Another reason to obtain a large amount of protein is the crystallization of protein and study its three -dimensional structure. Biochemistry of proteins can be difficult in any system, but it was particularly difficult to do with human proteins before the arrival of recombinant human proteins.