What Are the Different Compound Microscope Parts?

Observation and research of biological sections, biological cells, bacteria and living tissue culture, liquid sedimentation, etc. At the same time, other transparent or translucent, powder, fine particles and other objects can be observed. Can be equipped with photography camera.

Binocular biological microscope

Observation and research of biological sections, biological cells, bacteria and living tissue culture, liquid sedimentation, etc. At the same time, other transparent or translucent, powder, fine particles and other objects can be observed. Can be equipped with photography camera.

Chinese name

Binocular biological microscope

Foreign name

binocular biological microscope

is widely used in

Biology, cytology, histology

Features

Electric light source, lighting can continuously adjust brightness

Eyepiece

Eyepiece tube can rotate 360 degrees freely.

Frame

Adopted a series of convenient and comfortable designs

Stage

Adopt hard and wear-resistant surface with high hardness and corrosion resistance

Binocular biological microscope is widely used in the research of biology, cytology, histology, medicinal chemistry, etc. Binocular biological microscope can perform three routine inspections in medicine and can be used for teaching in school laboratory. The binocular biological microscope uses an electric light source, the illumination can continuously adjust the brightness, and the eyepiece tube can rotate 360 degrees freely.

Binocular biological microscopes are widely used in school teaching, hospital teaching, and home education. The instrument is small in size, light in weight and easy to operate. Binocular biological microscope is suitable for medical institutions, laboratories, research institutes and institutions of higher learning.

I think most users are concerned about what microscope to choose to truly meet my needs. Now we mainly focus on inverted microscopes to tell you when to use inverted microscopes. The sample used by the general user is in a cell culture plate or a petri dish (such as the 96-well plate mentioned in your question). The plate has a certain thickness. If this thickness is greater than the distance required by your magnification, the objective lens cannot It is close to the sample and cannot be observed. At this time you will need to use an inverted microscope. The advantage of an inverted microscope is that the order from top to bottom is: sample, stage, objective lens. In this way, the objective lens can be close to the sample without being limited by the thickness of the sample.

What do customers care about most about inverted microscopes?

First, let's talk about the main parts of the inverted microscope, which are also factors that customers need to consider when choosing.

1 : Optical part : The core of microscope quality is its optical part, namely the eyepiece and objective lens . For objective lenses, there are generally several levels. The first is an achromatic objective lens. With this objective lens, not all areas of the imaging are clear, only about 60% of the center of the field of view is clear, and there are certain defects in the outer 40%. Usually we place the observation part in the center of the field of view, so it does not affect the observation. But if you want 100% field of view without defects, you need to use a plan achromatic objectives. Relatively speaking, this price will be much higher, generally used in medical and scientific research. There is something in between, which is a semi-flat achromatic objective. Students use the microscope to select the first two levels. Generally cheaper objective lenses are ordinary achromatic objectives, and the transaction prices are around 8,000 yuan. The better ones are apochromatic objectives or infinity achromatic objectives, with transaction prices of around 10,000 yuan. If it is three-headed, the price is a few thousand yuan higher. Therefore, it is relatively simple for users to purchase eyepieces, that is, to see the size of their field of view, it is recommended to use eyepieces with large fields of view, because the eyepieces with large fields of view have larger openings than ordinary ones, and the visual effect is better. However, under normal circumstances, customers choose to use an inverted microscope for three purposes.

2 : Structural materials : For example, the fuselage structure of the microscope has a long service life. Some can reach more than 10 years. Then high-quality components and strict assembly become the guarantee of its service life. Therefore, a microscope with a sturdy frame should be selected, and the material is best selected to be alloy, which can reduce deformation. In addition, the size of the stage is also very important. Generally, the larger the stage, the higher the price of the microscope, because the larger the stage, the larger the sample port, the larger the observation area, and the easier it is to move the sample. .

3 : Light source part : Microscope observation can include bright field, dark field, polarized light, phase contrast, fluorescence and other light sources. Different function microscopes use different observation methods and have different functions.

Bright field image (BF): The image (transmitted beam) formed by direct electrons is used, and the image is clear. Generally bright field light source is the most common light source

Dark field image (DF): An image (diffracted beam) formed by scattered electrons is used, which has distortion and low resolution.

Many customers do not understand the dark field light source. Here we explain in more depth what the dark field observation is for specific dark field characteristics: Dark field observation (Dark field): The dark field is actually dark field lighting. Its characteristics are different from the bright field of view. It does not directly observe the illumination light, but observes the light reflected or diffracted by the object. Therefore, the field of view becomes a dark background, and the object under inspection appears bright.

The principle of dark field of vision is based on the optical Dondall phenomenon. The human eye cannot observe the dust when the strong light passes directly, which is caused by the strong light diffraction. If the light is directed obliquely to it, the particles appear to increase in volume due to the reflection of the light and are visible to the human eye. A special accessory required for dark field observation is a dark field condenser. Its characteristics are not to let the light beam pass through the object from bottom to top, but to change the path of the light to make it obliquely toward the object to be inspected, so that the illumination light does not directly enter the objective lens. Bright image. The resolution of dark field observation is much higher than that of bright field observation. Polarized light with a maximum of 0.02-0.004 is used to study the so-called transparent and opaque anisotropic materials. All materials with birefringence can be clearly distinguished under polarized light observation. Of course, these materials can also be observed by staining, but some are not possible, and polarized light must be used. Generally, the family of polarizing microscopes is 5,000 to 8,000, and the expensive ones are 20,000 to 30,000. Also, it is best to use monochromatic light when purchasing a polarizing microscope, because the speed, refractive index, and interference of light differ depending on the wavelength. General microscopy can use ordinary light. As for the phase contrast light source, it has already been introduced.

Fluorescent light source: It uses a certain wavelength of light to excite the specimen to emit fluorescence. It is magnified by objective and eyepiece systems to observe the fluorescence image of the specimen. Therefore, most of the fluorescent light sources are used on fluorescent microscopes.

4 : The number of meshes of inverted microscopes: Normal customers of inverted microscopes often use three objectives. In fact, they are dual-purpose. Single objectives are rarely used on inverted microscopes. It should be said that there are almost none. Therefore, many domestic manufacturers only produce The price difference between binocular and trinocular and general binocular and trinocular inverted microscope is about 2,000 yuan.

The above are some of the first considerations for customers when choosing a microscope. We will talk about some products and prices of mainstream domestic manufacturers of microscopes, as well as the microscopes that we routinely configure for customers.

It s better to invert this one. It s still an imported product. If customers have enough money, it s best to buy imported ones (such as Olympus in Japan or Nikon in Japan, CarlZeiss and Leica in Germany). If the funds are not enough, You can also buy domestic products. There are still many good manufacturers of inverted biological microscopes in China. For example, Chongqing Optical's inverted microscopes are generally accepted in the field of biomedicine. There are also Jiangnan Yongxin and Shanghai Optics' Shanghai Leader. The finished products are good. Chongqing Optical XDS-1B inverted biological microscope usually sold at about 12,000, and Jiangnan Yongxin XD-202 inverted biological microscope direct user transaction price: about 14,000 imported products are relatively low, generally 40,000 ~ 80,000 to 100,000 for 60,000 expensive

The products that Shanghai Tiancheng Technology Co., Ltd. often configures for customers are as follows: 400-700-0101

XDZ-103CE inverted biological microscope + C-type interface + 3 million color digital camera (with measurement software) = 11500 yuan / set

I have already told you how to choose a microscope, the scope of application, and the working principle. Now we talk about the after-sales issues and installation and adjustment issues that customers are most concerned about, as well as the main consumables and accessories of the instrument.

Everyone knows that this microscope belongs to an optical instrument. In fact, the biological microscope among microscopes is the simplest and the earliest invention of the microscope. His technology has been used for hundreds of years, so it has matured in technology and appeared. The possibility of failure is very low. Generally, his overall support generally uses Lu alloy racks or steel racks, plastic racks and light metal racks. The normal service life is relatively long, so the general microscope will not be covered by the warranty. What went wrong.

As for the operation of the microscope, most people learned from the middle school teachers. Although the use of the microscope is relatively complicated, it still requires a certain amount of patience. According to the instructions in the instructions, the operation is relatively simple. But before use, how to install a newly purchased product is also a problem. Some customers need to view biological samples through a computer or TV, or want to take pictures, save, write articles, and then you can add a variety of CCDs, software, or phase contrast devices, and other accessories on the microscope, this time involves installation and adjustment Now, the general configuration situation like this, the manufacturer will also provide you with on-site service. So customers can buy with confidence.

Imported biological microscopes / system microscopes: The imported microscope medical flow mall mainly deals with the brand of Olympus in Japan. Olympus in Japan does a better job in imported microscopes, and accounts for about 50% of the entire market in China. The price of imported microscopes is relatively low, and Olympus's microscopes are relatively complete in after-sales, so the prices quoted generally include installation and adjustment costs. One year warranty and lifetime maintenance. If the customer's budget is sufficient, it is generally recommended to buy imported microscopes, no matter from the after-sales and product materials, it will definitely be better than domestic microscope manufacturers.

The main accessories of the microscope are: computer, camera, camera (different pixel prices are different), software, capture card, phase difference device, camera connector and other major accessories

The main consumables of the microscope are (relatively easily damaged parts): objective lens, eyepiece, oil, halogen lamp, excitation block, tungsten lamp, fluorescent lamp, bulb.

After-sales service: We promise one-year warranty and lifetime maintenance after sales

If you have more questions about the choice of instruments, you can consult the medical flow mall 400-700-0101

Biological microscope / Inverted biological microscope / Stereo microscope installation and debugging / Olympus maintenance / Fluorescence microscope / Metallurgical microscope / Microscope price /