What is a Bioreactor?

A bioreactor refers to a reaction system inoculated into a liquid phase or a solid phase using naturally occurring microorganisms or microorganisms with special degradation capabilities. The two types of reactors that have been researched the most are the "elevator reactor" and the "soil slurry reactor". The lifting type reactor is to provide proper nutrition, carbon source and oxygen through the flow of water phase, so as to achieve the purpose of degrading pollutants in the soil. Compared with solid-phase systems, bioreactors can effectively degrade pollutants in a shorter period of time. The bioreactor technology has been applied to the bioremediation of organic contaminated soil. ^[1]

Bioreactor

A bioreactor refers to a reaction system inoculated into a liquid phase or a solid phase using naturally occurring microorganisms or microorganisms with special degradation capabilities. The two types of reactors that have been researched the most are the "elevator reactor" and the "soil slurry reactor". The lifting type reactor is to provide proper nutrition, carbon source and oxygen through the flow of water phase, so as to achieve the purpose of degrading pollutants in the soil. Compared with solid-phase systems, bioreactors can effectively degrade pollutants in a shorter period of time. The bioreactor technology has been applied to the bioremediation of organic contaminated soil. ^[1]

Bioreactor is a device system that utilizes the biological functions of enzymes or organisms (such as microorganisms) to perform biochemical reactions in vitro. It is a biological function simulator, such as a fermentation tank,

Name: Bioreactor

Subject Keywords or Keywords: DNA Life Science Bacteria

In 1993, Dr. Sang of the Roslyn Institute in the United Kingdom studied the expression system of yolk in poultry and expressed exogenous in the yolk

Biological Medicine for Prevention, Diagnosis, and Treatment of Foreign Medicine, Volume 22, Issue 5, 1999

Keywords: transgenic animal bioreactor drug gene construction expression

Abstract In recent years, achievements in the fields of biology and molecular biology have promoted the vigorous development of transgenic animal bioreactors. The use of genetically modified animal bioreactors to produce pharmaceutical proteins is another revolution in the field of biotechnology. It is different from the production of traditional medicines in a new mode of producing precious pharmaceutical proteins. This article focuses on the genetic construction of transgenic animal bioreactors and transgenic animals

Currently, the most typical example of protein expression in homologous tissue is the expression of human hemoglobin in the red blood cells of animals. In the human hemoglobin gene coding sequence, the promoter has two CACCC boxes, while the corresponding pig has only one promoter, and the other near it is the CGCCC box. Sharma et al. [1] encoded pig -promoter with human

Recombinant proteins expressed by transgenic animals mostly target breast, salivary and bladder. In these expression organs, by constructing appropriate vectors, selecting appropriate promoters and regulatory sequences can produce recombinant proteins that are much higher than normal levels. However, the production system should be isolated from the circulatory system as much as possible to reduce the effect of the expression product on the host animal.

The required target gene is constructed into a vector, and appropriate regulatory sequences are added to the animal embryonic cell, so that the milk secreted by the transgenic animal contains the required medicinal protein. There is a process from the transfer of the fusion gene to the embryonic cells to the collection of proteins, including

Low cost

2. Simple equipment

3.High efficiency

4. Significant product effect

5. Reduce industrial pollution

1. Improve milk quality;

2. Production of medicinal proteins.

3. Site integration of foreign genes in animals;

4. Tissue specificity of milk protein gene expression;

5. Post-translational modification of the target protein;

6. Isolation and purification of transgene expression products;

7. Technical and methodological issues of genetic modification;

8. Ethical issues.

Bladder bioreactor

The bladder reactor has the same advantages as the mammary gland reactor: it is easier to collect the product protein, and it does not need to cause harm to the animal. In addition, the system collects products from the time the animal is born, regardless of the animal's sex and whether it is in the reproductive period. The most significant advantage of the bladder bioreactor is that protein extraction from urine is simpler and more efficient than extraction from milk.

Bladder bioreactor uses Uroplakin promoter to start human

Bioreactors have gone through three stages of development: bacterial genetic engineering, cellular genetic engineering, and transgenic animal bioreactors. The emergence of transgenic animal bioreactors has attracted much attention because it overcomes the shortcomings of the former two, that is, bacterial genetic engineering products often do not have biological activity and must pass through

A bioreactor is a device that enables biological reactions. There are various forms of bioreactors. To make bioreactors run well, we must first have a deep understanding of bioreactors and reaction characteristics. This is the concept of bioreactor engineering. Bioreactor engineering focuses on the characteristics of the bioreactor itself, such as its structure and operating mode, and the relationship between operating conditions and cell morphology, growth, and product formation. It is combined with biological response engineering to solve the problem of selecting the best bioreactor and optimal operating conditions for various biological reactions.