What Is a Calibration Curve?

When using the calibration curve, please note that the calibration curve includes "standard curve" and "working curve". When applying a standard solution to make a calibration curve, if the analysis step is omitted compared to the sample analysis step, the prepared calibration curve is called a standard curve. If the composition of the analyzed substance is simulated and the analysis process is exactly the same as that of the sample, then the calibration curve drawn is called the working curve. Therefore, if matrix effects are critical to the analytical method, calibration curves should be drawn using a series of standard solutions containing a matrix similar to the actual sample.

Calibration curve

Care should be taken when using the calibration curve: the calibration curve includes "

Calibration curve

Under the specified conditions, the measured value and the actual instrumentation

(1) For the standard series, after the solution is measured with pure solvent as a reference, a blank correction should be made first, and then a standard curve is drawn;

(2) The standard solution can generally be directly measured. However, if the pretreatment of the sample is complicated and the contamination or loss cannot be ignored, it should be treated after the same treatment as the sample. When working curve should be made.

(3) The slope of the calibration curve often changes with changes in test conditions such as ambient temperature, reagent lot number, and storage time. Therefore, it is most ideal to draw a calibration curve while measuring the sample, otherwise two samples of zero concentration and medium concentration standard solution should be measured in parallel while measuring the sample, and the average value should be subtracted from the corresponding point on the original calibration curve. Check the relative difference according to the method precision should not be greater than 5% -10%, otherwise the calibration curve should be redrawn. ^[1]