What is quantitative mass spectrometry?
Quantitative mass spectrometry is a method of determining the molecular weight of the compound and from what is made. The mass spectrometry works by exposing the sample to extreme conditions of heat and electricity, causing it to penetrate into charged molecular fragments. The composition and abundance of these fragments is analyzed to reveal matter and composition.
There are many types of quantitative mass spectrometry, but each method uses the same set of processes. The sample is first heated to form steam, then ionized and accelerated by means of an electric field. Each has a single positive charge and these charged particles are diverted by their passage through the magnetic field. Lighter ions are diverted by more magnetic field than heavier ions, so the changing force of the field channels of ions of different matter into the detection device.
methane analysis, the simplest hydrocarbon - or a compound composed of hydrogen (H) and carbon (C) - reveals the presence of fragments that have atomic mass 1, 12, 13, 14, 15 and 16 atomic weight (AMU). Methane has a formula of ch 4 and methane sample analysis reveals the presence of H ++, ch + ++++++++++ + There is a very low abundance of fragment weighing 17 AMU on the weight spectrum for methane. This reading is due to the presence of an isotope of carbon or hydrogen. Isotopes are elements that have chemical properties but various atomic weighing because they have different numbers of neu in their nucleitron. The nucleus of the carbon-12 contains six neutrons and six protons, but much more rare carbon-13 isotope contains seven protons. Similarly, a small amount of hydrogen hydrogen-2 hydrogen, also called deuterium, which has a core of one proton and one neutron. as well as the analysis of the composition of organic compounds and the relative abundance of isotopes in the sample is also used to clarify the composition of biological molecules such as proteins, as well as quantitative mass spectrometry. The proteins consist of an amino acid chain or sequence and weight spectrometry can be used to determine the sequence in which these amino acid residues occur. Molecular weight of protein can also be found by quantitative mass spectrometry. ++++ The abundance of these fragments is also measured, with the highest being at 16 AMU, which corresponds to the weight of the uniform ion. This is because the removal of hydrogens from central carbon requires extreme energy, which means that the most abundant ion will be the most energetically favorable.