What is an enzyme -connected immunosorbent?
The enzyme immunosorbent test (ELISA) is a type of test used to measure antibodies or antigens in a fluid sample. Elisa is also known as an immunoanalysis enzyme (EIA), biochemical technique that is commonly used in immunology. It is used to diagnose conditions that may cause the reaction of the immune system. Enzymes are used to indicate antigens or antibodies to determine the type of disease, illness or allergy that a person has. Diseases such as cancer and autoimmune conditions can be detected by an enzyme -bound immunosorbent. The ELISA test can be used to target specific antibodies, which is produced by the immune system in the fight against a foreign invasion. ELISA tests are also used to determine specific allergies that a person may have.
The enzymes that are used for an immunosorbent test associated with the enzyme are specific proteins that cause reaction with the antibodies or antigens they are focused on. Sample's blood is taken and added to the test plate, ktErá has several wells in the columns. In these wells, there is first a solution that contains antigens specific to the condition, with a blood sample at the top. The primary antibody is added to both fluids that bind to antigens. Secondary antigens are added that bind to primary antibodies, followed by an enzyme.
As soon as the enzyme is added, the enzyme -bound immunosorbent test begins to change colors if the sample contains a specific antigen or antibody associated with the condition. Initial color changes show that primary and secondary antibodies are joined. When the color has changed, the results are considered positive. The positive result shows the presence of the state. In the ELISA test, it is common for multiple antigens to be used to exclude several conditions or allergies at a time.
There are various Ees color degrza color with positive results. The enzymed immunosorbent test can have a brighter or lighter color independence on the concentration of antibodies or antigens in the sample. For example, if the antibodies are higher than usual, the color change will be much brighter. With many conditions, these results can help doctors in determining severity.
enzyme -connected immunosorbent tests tests are sensitive tests and generally accurate. Although the Elisa test was first used to test the human immunodeficiency virus (HIV), it has many applications today. Generally, the ELISA test is used in the medical field, but can also be used in testing plant biology and industrial quality testing.
Radioimunoassay was a similar test that was used before the Elisa test. This version uses radioactive antigens and antibodies to test samples instead of enzymes. While some laboratories are still using this technique, most use the Elisapotože test is safer to play than its predecessor.