What is Puromycin?
Puromycin is antibiotics that scientists can use to work with cell cultures when they need a selective agent, a compound that kills all cells without resistance genes, with only specific goals. This antibiotic is available through laboratory suppliers and is only suitable for research purposes. People can order it in different concentrations and sizes, depending on their needs, from companies capable of producing. This causes cells to die if they do not have a gene for resistance, allowing them to survive the exposure of antibiotics. Scientists can indicate specific types of cells with this gene and grow them in culture using puromycin to create a pure culture. Any contamination will be eliminated by an antibiotic. Selective substances are useful for genetic engineering and other types of research where people want to be able to isolate specific types of cells.
People CPR CHECK Antibiotic into the culture medium and prepare a culture boardTo select before adding cells that intend to grow. The medium provides nutrition for cells so that they can grow and divide and create a cluster of cells for a researcher to work with. The use of antibiotics in the medium maintains undesirable cells and organisms; For example, if bacterial contamination occurs, puromycin will kill it because it will be susceptible to antibiotics.
Although many people associate antibiotics with drugs that people can use to treat infections, puromycin is not safe for using human or animals. It damages the cells in the body using the same inhibitory processes it uses in culture, and can potentially cause serious illness if people have taken a large dose. For this reason, the packaging has detailed warning labels that highlight people to the fact that they should not use it in medical treatment and companies that produce puromycin and drugs are cautious to insulate, andwould prevent contamination.
Scientists can choose from a series of selective substances when they prepare cells for culture and grow specific cell lines. It is possible to buy cells already equipped with resistance genes for projects, sometimes through the same suppliers offering antibiotics. Scientists can use a laboratory protocol or their own protocol that develops on how to handle cells in culture to make sure that their results are consistent, reliable and replicable to publish and distribute results.