What is tissue insertion?

tissue recessed is the tissue preparation technique used when the technician must be able to cut very small tissue samples known as a microtome. The tissue is supported in the medium, allowing the technician to create even, accurate cuts without crushing or otherwise damage to the tissue. Tissue slices can be examined under a microscope to study their characteristics and collect information for use in diagnostic assessments and other studies. The technician places the tissue in the cassette designed to immerse in the insertion medium. Parafin, plastics, epoxides and some types of gel media can be used. After setting the media, the sample can be pulled back, checked to confirm that the insertion has been smoothly and sliced ​​is cut as needed. Thanks to the support of the media, very fine slices of tissue are available, allowing the technician visualizing a high level of detail.

Placing samples in a cassette requires a certain trainingAnd experience, because the tissue must place appropriately for the study. If it is not placed correctly, it will be difficult to reduce the desired cross -sections and the sample can be destroyed. Before insertion, the processing of samples according to the relevant protocol for the tissue and treatment type must also be paid. Most laboratories have their own tissue handbooks and other procedures and expect technicians to follow these manuals.

devices designed specifically for tissue insertion are available for laboratories that need such devices. These machines differ in size and design depending on the number of samples that are designed for processing. Some are designed for specific media insertion, including proprietary compounds designed for specific types of histopathological applications. Tissue folding UE tends to be expensive and manufacturers have sales representatives who can provide information and advice when the laboratory selects new or replacement equipment.

sliced ​​tissue parts can be namont on the slides and examine or store in the storage. If part of the sample is left intact, it will also be stored after inserting the tissue if further tests are needed in the future. Stored samples can also be used for scientific research. The sliced ​​samples can be colored to emphasize certain functions or look for traces of specific chemicals and other compounds.

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