What Is a Primary Neuron?
The technology used in the primary cell nucleic acid transfection instrument is the patented Nucleofector technology of German amaxa company. Its principle is to use a unique electric field parameter and a cell type-specific transfection solution to directly transfer DNA into the nucleus. Even for primary cells (including non-dividing blood cells or neurons), the expression of transfected genes can be detected 2-4 hours after transfection. High transfection efficiency and simple and convenient operation.
Primary cell nucleic acid transfection instrument
Right!
The technology used in the primary cell nucleic acid transfection instrument is the patented Nucleofector technology of German amaxa company. Its principle is to use a unique electric field parameter and a cell type-specific transfection solution to directly transfer DNA into the nucleus Even for primary cells (including non-dividing blood cells or neurons), the expression of transfected genes can be detected 2-4 hours after transfection. High transfection efficiency and simple and convenient operation.
- Chinese name
- Primary cell nucleic acid transfection instrument
- Foreign name
- Nucleofector II Manual
- Function
- Transfect DNA, RNA or siRNA
- operating
- Easy and fast
Nucleofector II Manual
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NucleofectorR II nuclear transfection instrument: · Optimized electroporation technology; · Unique electrotransfection program: each program is adapted to different Cell type. | NucleofectorR kit: · Cell-specific transfection reagents; · The only nuclear transfection reagent; · Patented pmaxGFP reporter plasmid | |
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NucleofectorR 96-well shuttle nuclear transfection system: Complete NucleofectorR 96-well shuttle transfection system Consists of three parts: Nucleofector II nuclear transfection instrument, 96-well Shuttle, and a computer with supporting software. Customers of the Nucleofector II transfection instrument can upgrade to high-throughput nuclear transfection simply by adding a 96-well Shuttle and a computer platform. | | 96-well NucleofectorR kit: · Removable 6 2 × 8 combination Nucleocuvette boards, 16 holes / bar; · High flexibility and repeatability; · High-throughput detection; · High security; Easy and fast operation |
For transfection of primary cells and cell lines that are difficult to transfect, such as neurons, T cells and PC-12 cell lines, etc., the DNA can be directly transfected into the nucleus for high efficiency transfection.
Product performance description
A, revolutionary transfection technology that directly introduces foreign genes into the nucleus
B, non-viral mediated transfection technology that can efficiently transfect primary cells
C, transfection technology that can complete the whole process from experimental operation to result analysis in a few hours
D, Innovative Global Shared Cell Optimized Transfection Procedure Database
4. Principle of Nucleofector Nuclear Transfection Instrument
AMAXA nuclear transfection technology is a non-virus-mediated transfection method. It combines traditional electroporation technology and patented cell-specific nuclear transfection solution to adjust and optimize electrotransformation parameters for different cell types (memory optimized transfection program) Directly introduce foreign genes into the nucleus of primary and passage cells. Compared with other transfection techniques, the Nucleofector nuclear transfection apparatus is particularly suitable for transfecting primary cells and difficult-to-transfect cell lines, such as T cells and PC-12 cell lines. Nucleofector technology is unique because it can directly transfect DNA into the nucleus, enabling efficient transfection of primary cells that are restricted by cell division. With the Nucleofector nuclear transfection instrument, high-efficiency gene transfection rates greater than 90% can be easily obtained in the fields of tumor research, immunology, tissue engineering, and cardiovascular disease research.
5.Nucleofector transfection procedure
Nucleofector's nuclear transfection instrument requires only four steps and is very simple and efficient.
Step 1: Process cells and count accurately
Step 2: Mix DNA, transfection solution, resuspend cells, add transfection cup
Step 3: Put the transfection cup into the transfection instrument, press the button to select the corresponding program, and press "start" to start transfection.
About 10 seconds.
Step 4: Remove cells and continue to culture
(1) High transfection efficiency
The transfection rate of most cell lines and primary cells is 50% -70%; the transfection efficiency of some primary cells, such as human epidermal fibroblasts, exceeds 90%.
(2) Fast transfection
Because gene transfection does not depend on cell division, the expression of genes can be detected in 2-4 hours in most cell types, greatly reducing the time required for traditional transfection methods to take 24-48 hours to express. This speeds up the experiment (it can be completed in 1 day).
(3) Simple and convenient operation
Nucleofector transfection technology can be completed in less than an hour.
Nucleofector nuclear transfection kit components:
a, cell-specific nuclear transfection solution
b, electric rotor
c, dedicated cell transfer tip
d, patented pmaxGFP reporter plasmid
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2, BMP12 induces rhesus monkey bone marrow mesenchymal stem cells to differentiate into tendon cells
3.Expression of CTLA4-EGFP fusion protein in K562 cells and its subcellular localization
4. Observation of the effect of non-viral vector-mediated neural stem cell gene transfection
Construction of 5, HLA-A 0201 and EGFP fusion protein expression vector and its expression and localization in K562 cells
Experimental study on co-transfection of bone marrow stromal cell-derived neural stem cells with recombinant plasmid pcDNA3.1his-hTH and pEGFP-C2
7, PD-L2 cleavage isomer and EGFP fusion protein expression and subcellular localization
8, Stable expression of human tyrosine hydroxylase transfected bone marrow neural stem cells
Efficient transfection of primary rat hippocampal neurons
10. Study on the expression of enhanced green fluorescent protein in human chondrocytes and the method for repairing and reconstructing human cartilage
