What Is Antisense RNA?

Antisense RNA

Antisense RNA refers to RNA that is complementary to mRNA and can inhibit the expression of genes directly related to the occurrence of disease. It blocks gene expression, has the characteristics of strong specificity and simple operation, and can be used to treat diseases and severe infectious diseases caused by gene mutation or overexpression. Antisense RNA can be divided into 3 types according to its mechanism of action: Class I antisense RNA directly acts on the SD sequence and / or part of the target mRNA

Antisense RNA refers to RNA molecules that are complementary to mRNA, and also includes RNA molecules that are complementary to other RNAs. due to

There are two sources of antisense RNA in cells: the first is

Classification and mechanism of antisense RNA: The table below summarizes

in

Since antisense RNA is in

With molecular biology and

[1] Long antisense RNA is not necessarily more effective than short antisense RNA;

[2] in

[1] When the 3 'end of antisense RNA has a stem-loop structure or a similar -independent terminator structure, it can stabilize the RNA molecule;

[2] Gorski et al. Also found that the stem-loop structure upstream of the 5 'end of the 32 mRNA of the T4 phage and the sequence nearby it can also stabilize the RNA molecule. Therefore, when designing an antisense RNA gene, it is best to clone the sequences that produce the secondary structure at the 3 'and 5' ends at both ends of the antisense RNA gene. Hirashima et al. (1986) found that antisense RNA targeting the SD sequence of the target mRNA and the AUG region was more effective than antisense RNA targeting the coding region alone. In 1989, Hirashima also found that antisense RNA targeting the SD sequence and its upstream region (but excluding AUG) was more effective. In eukaryotes, antisense RNA targeting the 5 'non-coding region is more effective. However, experiments have shown that antisense RNA targeting the first intron is equally effective.