What is an absorbent spectrophotometer?
Absorption spectrophotometer is an analytical device used to determine the amount of element present in the sample. It relies on the fact that the elements absorb light at a certain frequency in the light spectrum. When the atom is on the lowest energy - or to the ground - can absorb photon lights with a specific frequency and jump into a higher energy state by energy from the photon. It is therefore possible to detect the element in the sample and determine the amount of measurement present by the amount of light of the frequency that is absorbed by the sample. This technique is widely used in chemistry for elementary analysis.
typical components of the absorption spectrophotometer include light source; a chamber or cell in which the sample is exposed to light; monochromator, which is a device that allows you to select and isolate the frequency of interest; and a detector that measures the intensity of the lightforecvent. Since the amount of light absorbed at this frequency is proportional to the concentration of the element, the amount of element present can be calculated. Data can be displayed onthe screen and/or stored electronically.
light source for absorbent spectrophotometer usually emits light on a specific frequency absorbed by the element for which the test is performed. Since the absorption frequency of the element is the same as its emission frequency, the source can use this element to create light required frequency. This can be achieved by electric discharge in an inert atmosphere using an element as a cathode. Normally, different light sources are required to test different elements, but in some cases a cathode containing several elements is used to allow simultaneous testing of these elements.
In the most commonly used typeabsorption spectrophotometer, the sample - usually a solution - is converted into aerosol and introduced into a hot flame, which converts all ions of interest into atoms in their basic state to absorb light. The type of flame used depends on the element of interest. For many elements withE uses oxygen and acetylene flame; However, some metals may form oxides if there is sufficient oxygen in the flame. In these cases, a nexidizing flame can be used. Other methods include the use of the graphite furnace to warm the sample to a high temperature and electrical heating the sample in a thin carbon rod.
There is a wide range of applications for the absorbent spectrophotometer. A common application in geology is to determine the content of various metals in a sample of minerals. In the area of environmental protection, this tool can be used to check the levels of toxic elements in industrial waste water, river water and ground Wanebo to determine the levels of toxic heavy metals in fish. Similarly, it can be used to check the levels of different elements in blood samples - for example, basic elements such as sodium, calcium and magnesium, as well as toxic elements.