What is a contrasting microscope?

2 The use of a contrast microscope allows someone to clearly see, visualization of structures connected to the building on stage, and this type of microscope also allows observers to see cells and organisms while they are still alive and pass through their life cycles, which is a clear advantage. Looking at the ordinary microscope, these components are basically flooded, which makes it impossible to see them. One way to solve this problem is to dye the body, and the dye provides a contrast that causes these structures to pop up. However, it may be difficult to dye samples, and the process of dying can change the cellular structure in addition to killing the body, which is unsuitable for people who want to see things by microscope to better understand them. Depending on the refractive index of different parts of the cell, the light slows down as it passes, moves "off the phase", as is known. With a special filter known as a phase plate between the sample and the eyepiece to amplify this change, the scientist will be able to clearly see transparent structures without By was necessary to color the pattern.

The differences between a microscope with phase contrast and a common microscope may seem like night and day. The relatively boring image suddenly is teeming under the contrasting microscope suddenly and detailed fine structures of organisms and cells suddenly become visible when they were not before. Using contrast microscopy, scientists can monitor life cycles and can also study detailed anatomy of such organisms.

The inventor of phase contrast technology, Frederik Zernike, was recognized in 1953 as his efforts by the Nobel Prize. Contrast microscopy can be significantly more expensive than other types of light microscopy because it requires special equipment. As a result, microscopes with phase contrast are usually observed only in specialized environments such as research laboratories. Students on university scientific classes on V VY -schools and universities may have the opportunity to work with such microscopes for their research and learn more about the field.