What is a biochemical laboratory?
Biochemical laboratory is an area in which biochemist studies chemical processes in living organisms. Traditional biochemistry examines the chemistry of the reactions of catalyzed enzymes, but biochemical research has spread to cover the topics of signal transduction, cell transport and molecular interaction. One generally must have at least a university degree in the sciences to work in a biochemical laboratory. Jobs in the biotechnology industry usually apply better than academic positions. They will also have a special freezer at -94 ° F (-70 ° C) for long -term storage of proteins and tissues. Such devices will have centrifuges and access to ultracentrifuge. ICE machine is generally necessary for generating ICE to maintain enzymes and reagents of chilled and stable. Virtually all biochemical laboratories will have gel electrophoresis of supplies to investigate proteins along with the western blot. Usually UV-VIS spectrophotometer will be enough, but some laboratories require fluorescence spectrofotWarter for more specialized applications. More highly specialized laboratories can have more specialized spectrophotometers.
Other biochemical laboratories may have more specialized equipment such as specific chromatographic equipment. This type of technology separates molecules. For example, the laboratory may have a high -pressure liquid chromatography (HPLC) to separate peptides or perform enzyme tests on small molecules. Another type of tool it might have is gas chromatography (GC) system. This unit separates volatile compounds.
Protein biochemical laboratory is likely to have fast pressure fluid chromatography (FPLC) to clean a large amount of protein to study. It would have different gel matrices, with different chemical properties to be used with FPLC to separate proteins. There would be glass columns of different dimensions that would hold nuts.Protein biochemical laboratories generally have a cold room, so the proteins can be insulated and cleaned at low temperatures to maintain stable.
Many biochemical laboratories use molecular biology techniques to help with biochemical research. Such laboratories of molecular biochemistry often combine many techniques of biochemistry, such as the execution of Western blots, with techniques of microbiological laboratories. Genetic engineering techniques include DNA or RNA handling in microorganisms, so sterile conditions are required. Such a laboratory should wipe the sterile caducil with ethanol and has a germicidal lamp. It blows sterile air through its desktop.
The laboratory should agar , which forms a gel on which microorganisms grow. For the media there would be a number of other supplies and antibiotics for growing genetically changed microorganisms. This should be incubators and shakes that could warm up so that bacteria or yeasts grow. TooRidual is access to the autoclave, sterilize supplies for growth and handling RNA and destroy the recombinant material after completion of experiments.
Other items would be gel electrophoresee units for RNA and DNA separation. The laboratory of molecular biochemistry should access the polymerase chain reactions (PCR) for PCR responses. It would have a number of restrictive enzymes and various enzymes used in cloning and PCR work.
Medical biochemical laboratory would have many items of other Labs biochemistry, depending on its specialty. The difference would be in the source of material for study. It would be more likely to use cell culture techniques or to obtain material from stem cell cultures. Recombinant human proteins are of great value for medical biochemical laboratories.
Many of these techniques are highly requested for biotechnology and academic research facilities. Techniques of molecular biology and PCR reactions are desirable work skills as well asKnowledge of mammal cells. The championship of any of the chromatographic units is also plus when hunting employment in biochemistry. University students can take laboratory courses in these areas and, if possible, conduct research in the biochemistry laboratory, which is a good way to start handling these skills.