What Is a Histopathologist?

The book is divided into 13 chapters: conventional pathology technology, immunopathology technology, molecular pathology technology, cell culture technology, real-time quantitative PCR technology, biochip technology, apoptosis detection technology, flow cytometry technology, electron microscope technology, Laser scanning confocal microscope and its application, laser microdissection technology, tumor animal experiment technology and image analysis technology. ^[1]

Histopathological techniques

The book is divided into 13 chapters: General

Series Title: Modern Biomedical Research Technology Series

Chapter 1 General Pathology Techniques

Section 1 Fixation and Materials

Section 2 Tissue Sectioning Techniques

Section 3 Hematoxylin-eosin Staining

Section 4 Semi-thin sectioning technique of plastic embedded light microscope

Section 5 Special staining methods commonly used (cytohistochemical methods)

Chapter 2 Immunopathology Techniques

Section 1 Principles and Applications of Immunohistochemistry

Section 2 Precautions and Results Analysis of Immunohistochemistry

Section 3 Immunofluorescence Technology

Chapter 3 Molecular Pathology Techniques

Section 1 in situ hybridization of nucleic acid molecules

Section 2 in situ PCR

Section 3 fluorescence in situ hybridization

Section 4 Application of gene rearrangement analysis in the diagnosis of lymphoma

Chapter 4 Cell Culture Techniques

Section 1 Cell Culture Basic Knowledge

Section 2 Cell Culture Medium

Section 3 Detection of Biological Characters of Cultured Cells

Section 4 Related Research Methods in Cell Culture

Chapter 5 Real-Time PCR Technology

Chapter 6 Biochip Technology

Chapter 7 Apoptosis Detection Technology

Chapter 8 Flow Cytometry Technology

Section 1 Flow Cytometry Works

Section 2 Flow Cytometry Features, Influencing Factors and Development Trends

Section 3 immunofluorescence staining, specimen preparation and precautions for flow cytometry

Section 4 Flow cytometry detection and common graphics

Section 5 Flow Cytometry for Detection of Human T Lymphocyte Subsets

Section 6 Flow Cytometry for Detection of Peripheral Blood NK Cell Activity

Section 7 Flow Cytometry to Detect Oncogene Proteins

Section 8 Flow Cytometry to Detect Cell DNA Content

Section 9 Flow Cytometry for Detection of Multidrug Resistance Gene Proteins

Section 10 Flow cytometry to detect apoptosis

Chapter 9 Electron Microscope Technology

Section 1 Transmission electron microscope

Section 2 TEM Sample Preparation

Section 3 SEM

Section 4 SEM Sample Preparation

Chapter 10 Laser Scanning Confocal Microscopy and Its Applications

Section 1 Basic Structure and Working Principle of Laser Scanning Confocal Microscopy

Section 2 Basic Functions of Laser Scanning Confocal Microscopy and Experimental Preparation Before Use

Section 3 Selection of Fluorescent Probes and Preparation of Fluorescent Samples

Section 4 Application of Laser Scanning Confocal Microscopy in Medical and Biological Research

Section 5 precautions and common problems and solutions

Chapter 11 Laser Microdissection Technology

Chapter 12 Experimental Techniques for Cancer Animals

Section 1 Animal Model Overview

Section 2 Laboratory Animals Commonly Used in Cancer Research

Section 3 Methods of Reproduction of Tumor Animal Models

Chapter 13 Image Analysis Techniques

Section 1 Image Analysis Techniques

Section 2 Application of image analysis in pathology and precautions