What Are Aminoglycosides?

Aminoglycosides are glycosides formed by the amino sugar and aminocycloalcohol being connected through an oxygen bridge.

Aminoglycoside

Right!

Aminoglycosides are glycosides formed by the amino sugar and aminocycloalcohol being connected through an oxygen bridge.

Author Zhao Zhuang; Jun Junwei; Zhang Jingying; You Xuefu; Jiang Jiandong; Hu Changqin;

[Author] ZHAO Zhuang1, TONG Jun-wei1, ZHANG Jing-pu1, YOU Xue-fu1, JIANG Jian-dong1, HU Chang-qin2 (1.Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China; 2. National Institutes for Food and Drug Control, Beijing 100050, China)

Author Institute of Medicine and Biotechnology, Peking Union Medical College, Chinese Academy of Medical Sciences; China Food and Drug Research Institute;

Abstract: Aminoglycoside antibiotics have been one of the commonly used antibiotics in clinic for more than half a century because of their broad antibacterial spectrum and strong antibacterial ability. However, aminoglycoside antibiotics have strong ototoxicity and nephrotoxicity, and are ranked first among drug-induced deafness factors. In this study, three kinds of aminoglycoside antibiotics, such as gentamycin, neomycin, and streptomycin, were used as representative drugs to study their toxic effects on zebrafish embryo development and larval hair. Cell damage, and explored the relationship between the damage and hearing-related genes. The results showed that: The lethal effects of the three drugs were obviously concentration-dependent, and the order of their lethal effects was streptomycin> neomycin> gentamicin; 5 dpf (day past fertilization) of the three drugs Juveniles have physical imbalances and abnormal body positions, as well as abnormal changes in the structure of the ear capsule; hair cell staining experiments can be observed, the hair cells and nerve mounds affected by the three drugs have obvious damage and reduced number; related to the development of auditory organs The genes eya1, val, otx2, and dlx6a were all down-regulated as the concentrations of the three antibiotics increased. This study explored for the first time the correlation between the treatment of these three ototoxic aminoglycoside antibiotics with the zebrafish sac structure and auditory gene expression; and proved that the use of zebrafish to establish a simple, accurate, intuitive, and rapid method for detecting ototoxicity of drugs and The feasibility of the detection method ^[1]