What Is Antioxidant Treatment?

Anti-oxidation is the abbreviation of anti-oxidation free radical, English Anti-Oxidant. The human body continuously generates free radicals in the human body due to continuous contact with the outside world, including breathing (oxidation reactions), external pollution, radiation exposure and other factors. Scientific research shows that cancer, aging or other diseases are mostly related to the production of excess free radicals. Research on antioxidant can effectively overcome the harm it brings, so antioxidant is listed as one of the main research and development directions by health care products and cosmetics companies, and it is also one of the most important functional demands in the market.

anti-oxidation

In order to live, in addition to food to provide sufficient nutrition, but also need to breathe continuously to obtain oxygen,

(1) Natural astaxanthin: a powerful antioxidant, known as the king of anti-aging, its ability to scavenge free radicals: 6000 times the efficacy of vitamin C; 1000 times the vitamin E; and - 100 times as effective as carotene; 20 times as effective as selenium; 700 times as effective as anthocyanin; 320 times as effective as tea polyphenols; 10 times as effective as lycopene; 800 times as effective as coenzyme Q10; Grape seed extract is 240 times more potent; 200 times more potent than carotol; and 3000 times more potent than resveratrol. ^[1]

Analysis of antioxidant cosmetics ingredients:

Tocopheryl acetate: Yes

ORAC Antioxidant ORAC

ORAC is the abbreviation of Oxygen Radical Absorption Capacity. It is an evaluation method system for testing the antioxidant capacity. [5]

Now that science has proven the importance of antioxidants to the body,

Quantifying the oxidative effect is an urgent issue that needs to be addressed. Only when the antioxidant effect can be quantified can companies develop better products, gain government recognition, and prove to consumers the effectiveness of their products' antioxidants.

Before the ORAC method was introduced, due to the extreme complexity of antioxidant testing, the commercial community (extremely complicated experimental protocols cannot be applied by the commercial community. At that time, a rigorous antioxidant experiment often took months and was very expensive. It is unacceptable to the business community.) It is impossible to effectively and comprehensively test the antioxidant power of samples. However, business is the driving force for technological innovation, just like Apple.

ORAC anti-oxidation test includes: five kinds of human body's main active oxygen radicals: peroxyl radicals (including hydrophilicity and lipophilicity), hydroxyl radicals, peroxynitroso, singlet oxygen, and superoxide anion. Comprehensive analysis can effectively obtain the actual antioxidant capacity and distribution of the sample.

The ORAC antioxidant biological test is a higher level test scheme than the ordinary antioxidant test. Using human cells to effectively test the sample's antioxidant power (bioavailability).

ORAC has been evaluated by the AOAC as the standard test method for oxidation resistance and is the mainstream international test method.

Antioxidant other evaluation methods

Antioxidation is not only a concept. The antioxidative effect on living organisms can be quantified. As an animal experiment, after taking antioxidants for a certain period of time, the changes in the ester peroxidation product, malondialdehyde, and the liver are measured Changes in the activities of SOD and glutathione peroxidase GSH-PX. From the changes of the above two enzymes and MDA, the strength and effect of antioxidants were determined. As the human body is impossible to measure liver homogenate, you can measure the MDA in blood or urine, and the SOD and GXH-PX in blood to determine the antioxidant effect.

Oxidants in food have long attracted the attention of scholars at home and abroad, because:

Antioxidants in food can protect food from oxidative damage and deterioration.

It has anti-oxidant effect in the human digestive tract and prevents oxidative damage in the digestive tract.

After absorption, it can play a role in other tissues and organs of the body.

Certain antioxidant-derived extracts derived from food can be used as therapeutic drugs. The mechanism of action of antioxidants includes chelating metal ions, scavenging free radicals, quenching singlet oxygen, scavenging oxygen, and inhibiting oxidase activity.

In vitro evaluation method

Although there are many, it is mainly based on two categories: one is to evaluate the antioxidant capacity of the test object by measuring the ability of the sample to inhibit the oxidation of lipids; the other is to use the sample's ability to scavenge synthetic free radicals to reflect the resistance of the test object Oxidation activity. The antioxidant activity of antioxidants in food and biological systems is affected by many factors, including the distribution effect of antioxidants between water and oil phases, oxidation conditions and environment, and the physical state of the oxidized substrate.

1. Determination of resistance to lipid peroxidation

Lipids include a wide range and are the main components of biofilms. Unsaturated fatty acids in lipids can be peroxidized. During lipid peroxidation, free radicals such as L, LO, LOO- and LOOH are produced. These products will Damage biological cells. Therefore, being able to inhibit lipid peroxidation has important biological significance.

A Ferrous thiocyanate method FTC: The ferric thiocyanate (FTC) colorimetric method is based on the oxidation of lipids by the oxidation of Fe2 + to Fe3 + under acidic conditions, and then Fe3 + and thiocyanate ions can form Red complex with maximum absorption in 480-515nm. Usually, the absorbance at 500nm indicates the ability of a substance to resist lipid peroxidation. The smaller the absorbance, the stronger the substance's ability to resist lipid peroxidation.

B-thiobarbituric acid reactant TBARs method: It is a common method to evaluate the degree of oxidation of fats and oils. The final product after oxidation of oil or linoleic acid is mainly malonaldehyde. These peroxidation products interact with thiobarbituric acid to form a colored compound, which has absorption at about 530 nm.

2. Defining DPPH Capability

DP is an early-synthesized organic radical. It is often used to evaluate the hydrogen-supplying ability of antioxidants. It is very stable in organic solvents, purple, and has a characteristic absorption peak. When it encounters a radical scavenger, The lone pair electrons of DPPH are paired to make them fade, that is, the absorption value at the maximum absorption wavelength becomes smaller. Therefore, the DPPH radical scavenging effect can be evaluated by measuring the change in absorbance.

3 Determination of reducing power

The determination of reducing power is a method to check whether a sample is a good electron donor. A sample with strong reducing power should be a good electron supplier. The electrons it supplies can not only reduce Fe3 + to Fe2 +, but also react with free radicals. Determination of reducing power is a common method used to evaluate antioxidant activity.

4 LOX enzyme inhibition experiment

The lipoxygenase LOX is widely distributed in plants as a non-heme ferritin with a molecular weight of 90 to 100KD. This enzyme protein is composed of multiple polypeptide chains, and the trivalent iron ion metal prosthetic group is active, while the divalent iron ion metal prosthetic group is inactive. The main role in the body is to specifically catalyze the oxygenation reaction of polyunsaturated fatty acids containing cis and cis-pentadiene structures to generate hydroperoxides of polyunsaturated fatty acids with conjugated double bonds. The main substrates in living organisms are free unsaturated fatty acids released by glycerolipids (such as phospholipids). The main substrate in animals is arachidonic acid, and the main substrates in plants are linoleic acid and linolenic acid. The main product is peroxybenoyl fatty acid.

The above method is an in vitro and in vivo measurement and evaluation method for the application effect of antioxidants.