What Is Assisted Insemination?
Assisted fertilization technology is one of the main measures to treat some male infertility. It is an extension of in vitro fertilization technology. Because it needs to be completed with the help of a micromanipulator, it is also called micro-fertilization. Assisted fertilization technology is of great value for studying the fertilization and development mechanism of mammals. In addition, it is also of great significance for saving endangered animals and making full use of excellent breeding males.
Assisted fertilization
- Chinese name
- Assisted fertilization
- Foreign name
- Assisted fertilization technology
- Treatment
- Treating some male infertility
- Extend
- In vitro fertilization
- Assisted fertilization technology is one of the main measures to treat some male infertility. It is an extension of in vitro fertilization technology. Because it needs to be completed with the help of a micromanipulator, it is also called micro-fertilization. Assisted fertilization technology is of great value for studying the fertilization and development mechanism of mammals. In addition, it is also of great significance for saving endangered animals and making full use of excellent breeding males.
- At present, there are two methods of assisted fertilization: sperm injection and zona pellucida modification. It is an artificial technique that enables sperm and eggs to complete the fertilization process and overcome the defects that sperm cannot pass through the zona pellucida and yolk membrane in some cases. . The sperm injection method uses a micromanipulator to inject sperm directly into the perivitelline space or cytoplasm of the oocyte. The former is called subzonal insemination, and the latter is called intracytoplasmic sperm injection. Zona pellucida insemination has strict requirements on the number of sperm injected. First, sperm that are viable and have undergone acrosome reactions must be injected individually. If there are no acrosome reactions, the number of injected sperm can be increased. The advantage of this assisted fertilization technique is that it does little damage to oocytes, but polyseed insertion is the main reason restricting the development of this technology. Intracytoplasmic sperm injection assisted fertilization technology has no special requirements on sperm motility, morphology, and acrosome response. It only needs to inject a single sperm. In order to increase the fertilization rate, the eggs need artificial activation after injection.
- Assisted fertilization technology. The zona pellucida modification method uses physical or chemical methods to perforate, partially cut or tear open the zona pellucida of the oocyte, open the channel for the sperm to enter the perivitelline space, and then share the egg with a certain concentration of sperm Cultivate to complete the fertilization process. This assisted fertilization technique is suitable for sperm that have a certain ability to move but have acrosome insufficiency and cannot pass through the zona pellucida. Its advantage is that the damage to the eggs is small, but for animals that rely on the zona pellucida to prevent polyspermia from entering the eggs, it is easy to cause polysperm to fertilize and affect the embryos' continued development.
- At present, intracytoplasmic sperm-assisted fertilization as a method of treating male fertility disorders has been applied in many countries, and the number of IVFs obtained therefrom has exceeded 3,000. However, the technique of assisted fertilization with zona pellucida modified method has only been successful in mouse experiments and has not been used clinically.