What Are Human Primary Cells?

Primary cells are cells that are cultured immediately after being removed from the body. Some people call the cultured first-generation cells and cells within 10 generations collectively referred to as primary cell culture. Primary cells are not only widely used in basic research in molecular, cellular biology and biomedicine, such as proteomics, genomics, cell line (line) research, DNA, RNA and genetics research, etc., but also can be applied to today's popular biology Pharmaceutical industry such as drug screening, drug metabolism and toxicology research, cancer drug research, etc. Primary cells have an irreplaceable role in the field of biomedicine and have a broad market prospect.

Primary cells

Primary cell culture refers to culturing cells, tissues, and organs directly from the body. Therefore, more strictly speaking, it refers to the culture before successful passage. At this time, the cells retain the basic properties of the original cells. If they are normal cells, they still retain the diploid number. However, in fact, the first to tenth generation of cultured cells are generally referred to as primary cell culture. ^[1]

The most commonly used primary cell cultures are tissue block culture and dispersed cell culture.

Tissue block culture is to transplant the cut tissue block directly on the wall of the culture bottle, and add the medium for culture.

The general steps of dispersed cell culture are:

The animal tissues are taken out of the body and separated into individual cells (usually trypsin), and cultured in a suitable medium to allow the cells to survive, grow and reproduce (note that the entire process is sterile).