What are the different types of technology of recombinant DNA?

Recombinant DNA technology includes a group of methods that insert foreign acid deoxyribonucleic acid (DNA) into organisms, either for a genetic study or strengthening the original organism. Inserting foreign DNA can be performed both in simple prokaryotic cells and more complex eukaryotes, but in genetic analysis organisms are often involved in individual cells. Three separate methodologies are used to handle these individual cells: bacterial transformation, nebacterial transformation and introduction of phage. Each of these three methods achieves roughly the same and integrates foreign DNA into the host organism genome. Each method is done differently, so each has an application in different contexts.

One of the most common methods of recombinant DNA technology is bacterial transformation. Sometimes known simply as a transformation, it includes the stimulation of a specially prepared bacterial cell to occupy a foreign DNA and integrate the iPhi into the bacterial genome. E. coli, bacterIEs that can sometimes cause food poisoning are often used as hosts of this method because they are easy to grow and reproduce quickly. A large number of transformed bacteria can give scientists quick and easy answers to questions about specific genes. A common application for transformation of bacteria is to test genes for drug resistance and try to anticipate how it changes.

The second range of transformation is called nebacterial transformation. This recombinant DNA technology is almost identical to bacterial transformation, except that bacteria are not used as host cells. Nebacterial transformations are commonly used in eukaryotic cells such as yeast or plant cells. This type of transformation can be made by filming DNA fragments connected to small pellets directly into the cell nucleus or injecting DNA to cell nuclei using micruoscopic needles. Both of these mEtody is more invasive than bacterial transformations, but there are certain types of cells such as plant cells that will not easily pick up pieces of foreign DNA due to cellular structure.

The third type of technology of a recombinant DNA is an introduction to phage, which includes the use of specific types of viruses called phages, to injure foreign DNA into host cells. Viruses can carry either single -thread or two -seamful DNA, so they can be used to replace a single -threaded DNA at specific locations. Not all phages are able to carry foreign DNA and not all phages that can carry foreign DNA are able to infect bacteria. Some phages can carry DNA more efficiently than others.

Unlike the prevailing image in popular culture, the recombinant DNA technology in your heart is not a group of methods that create "unnatural" organisms. Instead, he uses common genetics among all organisms to obtain information that would be difficult to generate or impossible to generate another way. This information withThen he uses for direct or indirect improvement in human health. Human health has many advantages of technology of recombinant DNA, including rice fortified nutrients in areas affected by famine and new therapies to combat genetic diseases.

IN OTHER LANGUAGES

Was this article helpful? Thanks for the feedback Thanks for the feedback

How can we help? How can we help?