What Is a Bacterial Artificial Chromosome?
Bacterial artificial chromosome (BAC) refers to a bacterial chromosome cloning vector constructed on the basis of F-plasmid. It is commonly used to clone DNA fragments of about 150kb in size and can store up to 300kb bases. Correct.
Bacterial artificial chromosome
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- Chinese name
- Bacterial artificial chromosome
- Foreign name
- Bacterial artificial chromosome
- Nature
- Bacterial chromosome cloning vector
- Foundation
- F plasmid
- Bacterial artificial chromosome (BAC) refers to a bacterial chromosome cloning vector constructed on the basis of F-plasmid. It is commonly used to clone DNA fragments of about 150kb in size and can store up to 300kb bases. Correct.
- This plasmid mainly includes oriS, repE (controlling F plasmid replication) and parA, parB (controlling copy number). The BAC-based cloned vector has a low frequency of chimerism, high transformation efficiency, and exists in the bacterial body in a circular structure, which is easy to distinguish, isolate, and purify. It is mainly used for the construction of large-scale genomic libraries and related research on large gene clusters, and plays an important role in various biological genome projects.