What is ultraviolet spectroscopy?
ultraviolet spectroscopy, often combined with visible spectroscopy, is a technique that is used in scientific and industrial laboratories to determine that the wavelength of light absorbs chemical solution. This information allows the researcher to analyze the content of the sample solution. Ultraviolet spectroscopy is performed with a special device known as an ultraviolet visible spectrophotometer. This type of radiation can be characterized on the basis of its wavelength. The total range of these wavelengths is a scale known as an electromagnetic spectrum.
Wave lengths of visible light range from about 400-750 nanometers. The shorter and higher energy wavelengths lying just before the visible spectrum, from about 10-400 nanometers, are classified as ultraviolet. Ultraviolet spectroscopy determines the absorbance of the chemical, specifically in the ultraviolet part of the spectrum.
When light passes through a chemical solution, a certain amount of light is absorbed and a certain amount is transmitted - forlies through without being absorbed. The amount of light that is absorbed by the compound can be measured by means of a spectrophotometer and can be used to determine the concentration of the solution. Higher levels of absorption indicate a more concentrated solution.
Chemical compounds not only absorb light but also absorb light at specific wavelengths. For example, a solution that appears to be green is the transmission of green visible wavelengths and absorbing red and blue. Ultraviolet spectroscopy can measure absorbance and permeability over the visible spectrum in the ultraviolet range.
The spectrophotometer works by directing the beam of light through the cuvette or a clear tube that contains a sample solution. In ultraviolet spectroscopy, the cuvette of the quartz glass must be rather than plastic, because the plastics tend to absorb ultraviolet light. The detector on the other side of the cuvette converts the incoming worldElectric power supply that can be read by electronics in the device.
Ultraviolet visible spectrophotometers with double beam measure both in the ultraviolet spectrum and in the visible spectrum using two Cuvet, one of which holds the sample, and the other contains a reference solution. There are also two light sources: one light source generates visible light and the other generates ultraviolet light. The grape component divides the incoming light into two rays. One beam is powered by each cuvet and the detector reads the results.
Information from the detector is used to generate a graph that portrays a wavelength against absorbance. The vertices in the graph indicate wavelengths that have been strongly absorbed by the compound. Quantitatively, they also show the overall absorption of the solution.