What Are the Different Types of E. Coli Treatment?

Escherichia coli , also known as Escherichia coli, was discovered in 1885 by Escherich. E. coli is a conditional pathogen, which can cause gastrointestinal infections or urethral infections in humans and a variety of animals under certain conditions.

The rapid and accurate detection of E. coli in food has become a common concern. The method and analysis of E. coli detection in food are described below ^[2] .

E.coli fermentation

This method mainly involves the cultivation of E. coli on a medium at 44.5 ° C, which contains a fluorescent substrate and requires 24 hours of culture. The fluorescent substrate is then released, which needs to be performed with glucuronic acid, so that the culture medium can emit fluorescence under the irradiation of ultraviolet light. In this way, you can also statistically estimate the colonies in the original sample. The main steps include fermentation, separation culture, secondary fermentation, and microscope observation ^[2] .

E.coli filter

The main process of this method: add about 10 mL of sterile water to the filter, then mix some sterile water to clean the inner wall of the filter, then filter, and place the filter membrane in M-FC medium, between the two No air bubbles are allowed, and then sealed. The storage temperature is 44.5 ° C and the storage time is about 24 hours until the E. coli flora becomes blue or blue-green. Then record the data, estimate the number of bacteria in the aqueous solution of each unit, and then convert the amount of E. coli ^[2] .

E.coli plate counting

Pipette 1 mL of a diluted sample with a sterile pipette, which is similar to lactose and bile salt fermentation, and place it in a sterile petri dish.

Then add 10 mL of CDLJ JD chromogenic medium at 45 ° C and mix the solution in the petri dish uniformly. You can quickly rotate the petri dish, and after the solution solidifies, add about 5 mL ^[3] Then, quickly shake the culture medium so that it can evenly cover the surface of the plate. After it solidifies, turn the culture medium and incubate it at 37 ° C for about 24 hours, then observe the changes in its shape and color. In addition, two dilution mediums are set up in parallel. The step is to first dilute the sample. After dilution, the microorganisms can be dispersed into single cells, and then cultured under certain environmental conditions until they grow into colonies, and then calculate the large intestine. The number of bacilli was calculated by dilution and sample number ^[2] .

E.coli immunomagnetic beads

The main principle of this separation technology is to use magnetic beads as a carrier and antibodies, combine the antibodies and magnetic beads, and then complete the mechanical movement by magnetic technology to isolate E. coli. Compared with other methods to isolate bacteria, this method has certain advantages. This technology can improve the detection success rate of pathogenic Vibrio in the sample, and the immunomagnetic bead technology can treat different microorganisms in different strains. , And then greatly improve the detection efficiency ^[2] .

E. coli automated instrument detection

Mainly using the immune automated analyzer, this technology was produced and used in 1970. With the development and advancement of science and technology, automatic instrument detection technology is widely used, and it is very convenient to operate. It can save a lot of time, its degree of interference is small, it can save manpower and material resources, and it can improve the accuracy of detection. In the current stage of development, the application of automatic enzyme immunoassay systems is very extensive ^[2] .

ATP E.coli ATP bioluminescence

In the development process in recent years, bioluminescence technology is widely used, and it is a relatively fast technique for detecting microorganisms. In living cells, ATP is its common energy metabolism product, which can provide the energy required during the physiological activities of cells. And, this technology can maintain a certain content in a certain range in the body. The detection technology of E. coli in food can adopt the method of fluorescence photometry, because the reason why the organism emits light is the effect of luciferase, which produces a luminous effect. The substance originated from the firefly in North America and can catalyze the oxidation of fluorescein. However, the substance is unstable and can rapidly decompose the fluorescence. In addition, the process of obtaining the results of the detection technology is very fast, and the device is convenient to carry, which is very suitable for on-site detection ^[2] .