What Are the Medical Uses of Polyclonal Antibodies?

The animals used for immunization are mainly mammals and birds. Rabbits, sheep, goats, horses, mules, guinea pigs and mice are often selected. The choice of animal is often determined by the use and amount of the antibody, and it is also related to the nature of the antigen. If you want to obtain a large amount of antibodies, use large animals. If you want to directly label diagnostic antibodies, use this animal directly. If you want to obtain indirect labeled diagnostic antibodies, you must prepare antibodies from heterologous animals; if it is difficult to obtain Antigens, and the amount of antibody required is small, can be prepared using pure line mice; antibodies used in general laboratories, mostly rabbits and sheep.

Polyclonal antibody technology

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Antigen stimulates the body to produce an immunological response. A group of globulins that are synthesized and secreted by the body's plasma cells and have specific binding ability to the antigen. This is the immunoglobulin, an immunoglobulin with specific binding ability to the antigen. It's antibodies. An antigen is usually composed of multiple antigenic determinants. An antigenic determinant stimulates the body. An antibody produced by a B lymphocyte receiving the antigen is called a monoclonal antibody. A variety of antigenic determinants stimulate the body, and accordingly produce a variety of monoclonal antibodies. These monoclonal antibodies are polyclonal antibodies, and the antibodies produced in the body are polyclonal antibodies; the same type of antigenic determination Clusters can also stimulate the body to produce five types of antibodies such as IgG, IgM, IgA, IgE and IgD.

The animals used for immunization are mainly

For soluble antigens, for the purpose of enhancing its immunogenicity or changing the type of immune response, saving antigens, etc., adjuvant methods are often used to stimulate the body to produce a stronger immune response.

After the antiserum is harvested, add 1 / 10,000 thimerosal or 1 / 10,000 sodium azide for preservation, or add the same amount of neutral glycerin, aliquot into vials, store at low temperature below -20 ° C, and antibody titer within months to years No significant changes. Take care to avoid repeated freeze-thaw cycles. Antiserum can also be stored after freeze-drying.