What Is Minimum Bactericidal Concentration?
Minimum bactericidal concentration (MBC): The minimum drug concentration required to kill 99.9% (down 3 orders of magnitude) of the test microorganism.
- Minimum bactericidal concentration (MBC): The minimum drug concentration required to kill 99.9% (down 3 orders of magnitude) of the test microorganism.
Minimum bactericidal concentration
- Minimum bactericidal concentration (: The minimum drug concentration required to kill 99.9% (down 3 orders of magnitude) of the test microorganism. The MBC of some drugs is very close to its minimum inhibitory concentration (MIC), such as aminoglycosides. The MBC of some drugs Larger than MIC, such as -lactams. If the test drug has a MIC with a MBC 32 times that of the test microorganism, it can be determined that the microorganism has developed resistance to the test drug.
Minimum bactericidal concentration
- The effective antibacterial concentration drug solution of the drug and the corresponding concentration of the drug solution 0.1ml were transferred to a nutrient culture agar plate containing no drug, and the drug solution was gently pushed with a sterile inoculation ring, and the plate was placed in a 37 ° C incubator. Cultivate for 24 hours, observe the growth of bacteria, and count 5 less colonies in the petri dish as the minimum bactericidal concentration [1] .
Minimum bactericidal concentration
- The determination of the minimum bactericidal concentration is affected by many factors. Therefore, for the concentration of inoculum liquid used in the test, the chemical composition and properties of the culture medium (solid or liquid), pH value, osmotic pressure, ionic strength, cation and growth factor concentrations Environmental conditions (such as temperature, partial pressure of various gases, humidity, etc.) and quality control should have a corresponding understanding to determine whether the experimental method meets the requirements of the standard, and whether it can also be used for clinical susceptibility testing of clinical isolates.
Minimum bactericidal concentration
- The minimum bactericidal concentration is an important indicator reflecting antibacterial activity, understand its experimental method and quality control, determine whether the experimental method meets the standard requirements, analyze the experimental results and its value to clinical trials, such as the minimum bactericidal concentration to kill 90% of bacteria, Compare the relationship between bactericidal concentration and bacteriostatic concentration [2] .