What is a tandem mass spectrometer?
The mass spectrometer is a tool that is often used to measure the weight of molecules, which can help identify the compounds in the sample. The charged particles called ions are released from the sample and analyzed. Multiple analyzers are called tandem mass spectrometer (MS/MS). Usually capable of fragmenting molecules and ions, it is often used to identify proteins and other organic substances. The device may include single -type spectrometers or combine several of them; Sometimes two, three or four analyzers are sometimes incorporated. The first analyzer can be used to achieve this. Ions are often stabilized by the second analyzer before the molecules are exposed to gas. Molecular rainfall causes the particles to break into fragments that are detected by the third analyzer. While there are several methods for fragmenting molecules, sometimes one crusher is used with a quadrupole ion trap that exposes an electric field ions and ejects them from the frequency field.
The tandem mass spectrometer detector usually monitors ion activity. It then creates a signal that is sent to the computer system that stores the data. The graphic graph is generally used to indicate what is in the sample and what is the molecular weight of each substance. The graph can also show the amount of each type of molecule or protein in the sample. There are different types of detectors that can be used in MS/MS, which include photomultoplants designed to detect electromagnetic energy or electron multipliers, which are vacuum tubes that knock electrons for current amplification.
different types of experiments can be done using a tandem mass spectrometer. It is usually common monitoring of one mass in which the first analyzer allows for the type of Pčlánek through, while the other is looking for a particular fragment. Monitoring multiple responses includes monitoring different types of ion fragmentThe second analyzer. Both are often used in pharmaceutical studies, while experiments with a precursor of ion scanning generally help to detect ion losses and detect molecular components that are not known.
Tandem mass spectrometer can also be used as a metabolic screening tool. It was used to find newborn disorders related to proteins called amino acids. Blood, thyroid and metabolic conditions can be detected by analyzing blood samples with MS/MS in laboratories.