What Is an Electrospray Mass Spectrometer?

Electrospray mass spectrometry is a mass spectrometry method with an electrospray ionization system.

Electrospray mass spectrometry

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Electrospray mass spectrometry is a mass spectrometry method with an electrospray ionization system.

Electrospray mass spectrometry electron spray tnas: r apertrc} meiry ";) '} SMS is a mass spectrometry with an electrospray ionization system.

As follows: The sample solution enters the electrospray chamber through a very thin sample tube. Under the action of a strong electric field, the sample solution is broken into many small charged droplets at the outlet due to the separation of charge and electrostatic attraction. Under the action of the charged droplet, the charged droplet drifts towards the inlet of the mass spectrometer against the direction of the flow of the dry gas. The reversed dry gas quickly evaporates the droplet and increases the charge concentration on the surface of the droplet. When the Coulomb repulsion and the droplet surface When the tension limit is equal, the droplet will burst into smaller droplets until the droplet becomes very small, because the radius of curvature of the droplet is small, and its surface charge density is large. As a result, a very strong electric field is formed on the droplet surface. This electric field is sufficient to desorb ions from the droplets. The ions enter the first vacuum zone with a pressure of several Torr (2 Torr = 133.322Pa) through the glass capillary, where collision activation cracking can be performed to obtain fragments of sample molecules, thereby obtaining molecules Structure information. The molecular ions and fragment ions of the sample pass through a series of separators and electrostatic lenses and enter the mass analyzer for mass analysis. This electrospray mass spectrometry has high sensitivity, and the ionized molecules can have multiple charges. The generation of such multi-charged ions greatly expands the mass range that ordinary mass spectrometers can analyze, enabling the mass spectrometer to analyze molecular weights of hundreds of thousands of masses. Unit of protein molecule. ^[1]