What Is Immunodiffusion?
Immunodiffusion means that soluble antigens and corresponding antibodies diffuse with each other in agar medium, meet each other, and form a precipitation line at an appropriate ratio.
Immunodiffusion
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- Chinese name
- Immunodiffusion
- Definition
- Mutual diffusion meets each other to form a precipitation line
- Function
- Can be used for semi-quantitative detection of antibodies
- Types of
- One-way and two-way immunodiffusion
- Immunodiffusion means that soluble antigens and corresponding antibodies diffuse with each other in agar medium, meet each other, and form a precipitation line at an appropriate ratio.
- I. Definition:
- 2. Function: Perform qualitative analysis of antigens or antibodies based on the presence, shape, and location of the precipitation line. It can also be used for semi-quantitative detection of antibodies.
- The antibody titer can also be determined.
- Three types: one-way immune expansion and two-way immune diffusion.
- Fourth, experimental precautions:
- 1. The slide should be clean and the edges should not be damaged.
- 2. Spread the agar plate evenly and without bubbles.
- 3. The holes should be rounded and smooth, and the edges should not be broken.
- 4. When adding samples, try to avoid air bubbles or adding to the wells to ensure the accuracy of the results.
- 5. Note that the microsampler must be cleaned after each sample is added.