What Are Digital Transcribers?

Transcriptome broadly refers to the collection of all transcription products in a cell under a certain physiological condition, including messenger RNA, ribosomal RNA, transport RNA and non-coding RNA; narrowly refers to the collection of all mRNAs. Protein is the main bearer of cell functions. The proteome is the most direct description of cell functions and states. Transcription composition is the main method for studying gene expression. Transcriptome is the inevitable link between the proteome that connects genetic information of the genome and biological functions. Level regulation is currently the most studied, and it is also the most important regulation method for organisms.

The research object of transcriptome sequencing is the sum of all RNAs that a specific cell can transcribe under a certain functional state, mainly including mRNA and non-coding RNA. Transcriptome research is the basis and starting point of gene function and structure research.

RNA-Seq (RNA-sequencing) RNA-Seq, also known as transcriptome sequencing, is a newly developed technology that uses next-generation sequencing technology to perform transcriptome analysis.It can comprehensively and quickly obtain a specific cell or tissue in a certain state. Sequence information and expression information of all transcripts, including mRNAs encoding proteins and various non-coding RNAs, and expression abundance of different transcripts produced by gene alternative splicing. While analyzing the structure and expression level of transcripts, unknown transcripts and rare transcripts are also found, so as to accurately analyze the important issues in life sciences such as gene expression differences, gene structure variations, and molecular marker screening.

In addition, RNA-Seq can directly analyze the transcriptome of most organisms, because it does not need to know the genetic information of the target species, which shows a special advantage. Prior to the emergence of RNA-Seq, people had limited knowledge of the transcriptome. RNA-Seq has been shown to be efficient and fast, which has largely changed people's understanding of the transcriptome. RNA-Seq has successfully sequenced model plants such as maize (Zea ma ys), Arabidopsis thaliana, rice (Or yza saliva), tomato (SolanumlycoPersicum), etc. ^[2]

With the first Illumina Genome Analyzer x sequencer in the Asia Pacific region entered Tianjin

Acquired species or tissue

Sample requirements:
Purity requirements of sample: Total RNA OD value should be between 1.8 and 2.2; electrophoresis detection 28S: 18S is at least greater than 1.5;

Concentration of sample: The total RNA concentration is not less than 400ng / ul; the total amount of sample is not less than 15ug; the current latest sample library building requirement is reduced to 1ug, and the concentration is greater than 50ng / ul.

(3) Please provide the specific concentration, volume, preparation time, solvent name and species source of the total RNA sample. Please also attach QC data, including electrophoretic gel maps, spectrophotometry or Nanodrop instrument detection data. If multiple sample preparations are required, samples required for multiple sample preparations need to be provided.