What Factors Affect Sperm DNA?
Sperm DNA fragmentation detection is a process for detecting sperm quality. The degree of sperm DNA fragmentation is considered as a new index to evaluate sperm quality and predict fertility.
Sperm DNA fragmentation test
- With the increase of China's industrialization, environmental pollution has gradually increased.
- Sperm count, density, activity rate and
- Is an important indicator of sperm maturity.
- When sperm are damaged, their DNA is denatured into single strands by the action of acid. Acridine orange (AO)
- It can emit green fluorescence in the form of a monomer in combination with double-stranded DNA and red or yellow fluorescence in the form of a polymer in combination with single-stranded DNA. Therefore, immature and mature sperm can be distinguished by the intensity of green fluorescence. Sperm with a strength above the main group represents immature sperm with loose chromatin packaging.