What is Gene Synthesis?

Gene synthesis refers to the technology of artificially synthesizing double-stranded DNA molecules in vitro. It is different from oligonucleotide synthesis: oligonucleotides are single-stranded, and the longest fragment that can be synthesized is only about 100 nt. For double-stranded DNA molecule synthesis, the length that can be synthesized ranges from 50bp to 12 kb. Gene synthesis is a technique for synthesizing genes by artificial methods. It is one of the means of gene acquisition. Compared with the genes obtained from existing organisms, gene synthesis does not require templates, and therefore is not restricted by the source of genes.

Gene synthesis

The first human synthetic gene appeared in the 1960s ^[1]

1: The synthesis cycle is short, which can guarantee 100% of the sequence is correct

Second: Gene synthesis has greater flexibility, and can modify the gene's digestion site and gene sequence to facilitate downstream cloning and experiments.

Three: Researchers design according to their own wishes to obtain genes that are difficult to obtain or even do not exist in nature

Four: Gene synthesis genes can be codon optimized, so that genes can be well expressed in various biological expression systems

1: clone human or mouse antibodies or recombinant antibodies

2: Synthesis of different gene mutants, SNPS, or other mutants

3: Designing and synthesizing DNA vaccines

Four: Large-scale synthesis of cDNA for microchips