What is involved in measuring the levels of the enzyme?

Laboratory tests that measure the level of enzyme and enzymatic activity are known as enzyme tests. There are many types of measurement methods that measure the speed of enzyme activities and inhibition of enzymes. Enzymes are molecules that manipulate other molecules known as substrates, binding with them and chemically react to the production of more by -products. Tests that measure this activity are for different purposes, depending on the specific symptoms of the disease, and consider different steps in enzymatic processes. There are factors such as enzymes such as enzymes in a specific way according to temperatures in the body, and enzymes cannot tolerate high salt levels, as too much salts disrupt the ability of the enzyme to bind to other proteins. Many enzymes can only work in a small pH extent; Therefore, the pH values ​​in the body ntaké measure. High pH can completely inhibit enzymatic activities.

There are two main types of enzyme tests: continuous, with constant and discontinuous values ​​accepted, with chemical reaction and measuring the amount of substrate and by -products concentrations after some time. In continuous tests, NADH, which affects metabolism or NADPH, which affects the body's ability to react well with drug therapy, is used by spectrophotometric test because it is displayed under its ultraviotic (UV) rays. As the fluorometric tests are called, they define differences in fluorescence in some enzymatic substrates. Calorimetric tests measure the amount of heat during enzyme chemical reactions and chemiluminescence tests measure light produced during chemical reactions and can detect antibodies for the disease. In discontinuous Assays, radiometric tests can measure the absorption or release of radioactivity when proteins and substrate bind and chromatographic tests measure the formation of side productionsKTs of the enzyme-substrate bonds.

Some blood tests are often performed to test specific enzyme levels to determine heart damage. For example, there are a certain number of several proteins that will be present in the blood in higher volumes when, for example, the heart muscle is damaged. These tests must be carefully performed, as kidney disease can also lead to almost the same level of these same enzymes. Another condition known as malignant hypertension may lead to elevated levels of heart enzyme; Therefore, for determining the likely causes of high enzyme levels, tests other than enzyme tests will be required.