What is the microdisection of the capture of the laser?
Laser microdising (LCM) is a technique used in biological laboratories that allows technicians to remove the designated area or type of cells from the sample. A dehydrated cellular sample is placed at the microscope stage and a thin layer of thermoplastic material, such as ethylene -vinyl acetate, is placed on it. When the laser beam is focused on selected cells, the membrane layer melts with them and binding, and these cells are built into the film after removal. The process is also known as laser microdising (LMD). It allows the study of different parts of tumors and their development phases. This technique is also used for various types of genetic analysis and for studying complex brain and immunological cells. It can be used in conjunction with other experiments with DNA and gene expression.
LCM is not the only type of microdisection. Another type of laser capture microdisence only uses laser to separate cell samples. NeeIt xists no contact between the cells and any type of material and energy of the laser itself moves the microdized cells to the sample vessel. Another system removes undesirable ultraviolet cells that lasts longer, but also does not require the use of a polymer film.
Due to its ability to obtain pure cell samples for molecular analysis, laser capturing microdisection has been increased by various studies and changes. It has been found that the use of frozen tissue provides better results. The adhesives were used on microscopic slides, but instead of helping to connect cells, it is difficult to capture specific tissue samples. Moisture the presentness has a negative effect on the acquisition of cells on the film, so dehydrated tissue slices are preferred during this technique.
Despite its advantages, the microdisection of laser capture has several disadvantages. The cost of microscopes, computers and software can be high. It may also be difficult to see samples because the microscope slide is not usedOJ is a problem with many similar techniques. Scientists also find that they need more time to perform the process, but the cleanliness of the LCM samples still outweigh the benefits of other methods.