What Are the Different Methods of Bacterial Detection?

Food bacterial inspection refers to the detection of bacterial content in food to obtain bacterial indicators, which reflect the freshness or cleanliness of food, whether processing operations meet hygienic requirements, identify whether food is contaminated with pathogenic bacteria, and confirm microbial food The cause of poisoning and so on.

The rapid test strip refers to a method of using a paper sheet, a paper film, a film, etc. as a medium carrier, attaching a specific medium and a color-developing substance on the top, and measuring the microorganisms in food by the growth and color development of the microorganisms on the top. The development of the total bacteria detection paper began in the 1980s, and its main advantages are simplicity, practicality, economy, and strong operability. In recent years, test papers based on filter paper and Petrifilm from an American company have started to be widely used. ^[1]

Bioelectrochemical method refers to the method of measuring the charge generated or consumed by microorganisms through an electrode to provide an analytical signal. During the process of breeding and metabolism of microorganisms, the electrochemical properties of the culture medium such as current, potential, resistance, and conductance will change. Therefore, rapid detection of microorganisms can be achieved by detecting and analyzing changes in these electrochemical parameters. Common are: impedance analysis, potential analysis, current analysis and so on. Bioelectrochemical methods have the characteristics of fast, intuitive, simple operation, low cost of measuring equipment, and controllability of signals. ^[1]

Micro-colony refers to the micro-colonies formed on the solid-phase carrier in the early stage of bacterial growth and reproduction, which can only be observed with the help of a microscope. Microcolony technology is fast, economical, and practical. Its research began in the 1950s and quantitative measurement technology began in the 1970s. It has been reported abroad that this method is used for the rapid detection of total bacteria in water and food. Many scholars in China have also studied this. ^[1]

The analysis of gas chromatography and high performance liquid chromatography is applied to the detection of microorganisms, mainly based on the chemical composition of different microorganisms or their metabolites. The above chromatographic detection can directly analyze bacterial metabolites and cells in various body fluids. Fatty acids, proteins, amino acids, peptides, polysaccharides, etc. to determine the specific chemical marker components of pathogenic microorganisms to assist the diagnosis and detection of pathogens. ^[1]