What Are Myeloma Cells?
Myeloma cells are a tumor caused by the malignant proliferation of the B-lymphoid plasma cell system. It is characterized by the malignant proliferation of abnormal plasma cells (called myeloma cells) in the bone marrow, and secretion of certain humoral factors, causing abnormal activation of osteoclasts, causing osteolytic lesions, causing bone pain and bone destruction, and anemia , Renal impairment, abnormal immune function. Myeloma cells have the following characteristics: stable and easy to culture; does not secrete immunoglobulins or cytokines; high fusion rate; is a hypoxanthine phosphoribosyltransferase-deficient strain, and is a B that produces monoclonal antibodies The most ideal splenocyte fusion partner in lymphoma hybridoma technology. (Ding Mei) [1]
Myeloma cells
- Chinese name
- Myeloma cells
- Foreign name
- myeloma cell
- ability
- Infinite split ability
- Natural enemy
- B lymphocytes
- Features
- Continuous proliferation under in vitro culture conditions
- Myeloma cells are a tumor caused by the malignant proliferation of the B-lymphoid plasma cell system. It is characterized by the malignant proliferation of abnormal plasma cells (called myeloma cells) in the bone marrow, and secretion of certain humoral factors, causing abnormal activation of osteoclasts, causing osteolytic lesions, causing bone pain and bone destruction, and anemia , Renal impairment, abnormal immune function. Myeloma cells have the following characteristics: stable and easy to culture; does not secrete immunoglobulins or cytokines; high fusion rate; is a hypoxanthine phosphoribosyltransferase-deficient strain, and is a B that produces monoclonal antibodies The most ideal splenocyte fusion partner in lymphoma hybridoma technology. (Ding Mei) [1]
- After mammals are infected with antigens, corresponding B lymphocytes will form in the body. B lymphocytes can secrete corresponding antibodies to condense or kill these antigens. During the immune response of animals, each B lymphocyte can secrete a specific antibody. To obtain a large number of specific antibodies, the B lymphocytes capable of secreting the single antibody must proliferate in large numbers. B lymphocytes have the ability to produce a single antibody, but cannot proliferate in vitro; myeloma cells are a type of cancer cell that can proliferate indefinitely in vitro, but cannot produce antibodies. Therefore, the fusion of a B lymphocyte with a myeloma cell to produce a hybridoma cell will have the characteristics of two parental cells-it can continue to proliferate under in vitro culture conditions and can produce a certain specificity Antibodies.