What is hemoglobin electrophoresis?
Hemoglobin Electrophoresis is a blood test using protein electrophoresis. Doctors use it to determine different types of hemoglobin present in the patient's blood and the ratio in which different types occur. This test is usually performed to determine whether certain types of disease are present if the patient is a carrier for a particular condition and evaluates the effectiveness of hemoglobin disorders. More than 400 different types of abnormal hemoglobin have been identified, all with different levels of clinical significance.
To perform the patient's techniques, it takes a blood sample and sends it to the analysis laboratory. The principle of hemoglobin electrophoresis is that each type of hemoglobin has a different electric charge and can be distinguished appropriately. Doctors perform electrophoresis using some form of transport medium, usually a solid gel plate that has a pH prefix. At one end of the album is a set of wells, which was cut into a gel. Blood sample from the patient is introduced into wells along with koa ncontal sample of known charge and identity.
The medical staff then applies to the gel. Different types of hemoglobin migrate from holes on the cathode or negative end of the gel towards the anode or positive end. Hemoglobin travels during hemoglobin electrophoresis at different speeds and creates visually identifiable gel belts according to type. Different types of hemoglobin travel known distances and can therefore be easily identified by a pattern that appears. Different belts thickness indicate the amount of each particular hemoglobin.
Hemoglobin electrophoresis can be very important in determining abnormal hemoglobins that may have clinical consequences for the patient. For example, a state called Sickle Cell Anemia can be identified by this technique. August cell is indicated by the presence of hemoglobin (HBS). Hemoglobin electrophoresis can further identify whether the patient has in realTheness of the illness or is a carrier of properties that can be handed over to their children.
During the test, if the patient does not have a condition or property, no belt appears in the known charge. If the patient is a carrier of the properties of sickle cells, a mild belt is formed. For those who have a condition, a stronger belt is evident. The results can be further quantified using high -performance liquid chromatography, which shows the amount of HBS as a percentage.