What Is a Splenocyte?
Spleen cells, the spleen obtained from the donor, spleen tissue is crushed by mechanical or other methods to make free spleen cells and stored in isotonic saline to form a spleen cell suspension. The total number of cells in the spleen cell suspension is calculated by conventional blood cell counting. One adult spleen can prepare 551-2002 billion splenocytes. Spleen cell survival rate was determined by two methods, cone blue staining and phase contrast microscopy. The formulated spleen tissue suspension should be applied immediately, only for short-term storage, and the storage period should not exceed 6h.
Splenocytes
Right!
- Chinese name
- Splenocytes
- To source
- spleen
- Application range
- Clinical immunology
- Test leader
- Abramov
- Spleen cells, the spleen obtained from the donor, spleen tissue is crushed by mechanical or other methods to make free spleen cells and stored in isotonic saline to form a spleen cell suspension. The total number of cells in the spleen cell suspension is calculated by conventional blood cell counting. One adult spleen can prepare 551-2002 billion splenocytes. Spleen cell survival rate was determined by two methods, cone blue staining and phase contrast microscopy. The formulated spleen tissue suspension should be applied immediately, only for short-term storage, and the storage period should not exceed 6h.
example
- Experts from the Russian Institute of Clinical Immunology have found that if a mouse is implanted with spleen cells from other mice, it will gradually become more active than mice that provide spleen cells.
- Researchers put multiple mice into plastic boxes with a length of 100 cm and a width of 40 cm and a height of 40 cm, and observed the mice's running mode, route and standing times for 5 minutes. Based on multiple observations, the rats were divided into A very active group, B moderately active group, and C inactive group. Subsequently, researchers injected about 15 million splenocytes from a single mouse in group A into a single mouse in group C by intravenous injection. After 5 to 14 days, the mice in group C became increasingly active. Conversely, if a large number of spleen cells of group C mice were implanted in group A mice, group A mice would become increasingly unmovable. In addition, the researchers also carried out the mutual transplantation of spleen cells in the same group of mice without changing their behavioral activity.
- According to Abramov, an expert in charge of the study, by analyzing the results of the experiments, they believe that there is a link between the animal's immune system and its advanced nervous system, and this connection can affect the activity of the advanced nervous system.