What Is a Cloning Vector?
Cloning vectors usually use DNA obtained from viruses, plasmids or higher biological cells as cloning vectors, and insert exogenous DNA fragments of appropriate size on the vectors, taking care not to destroy the self-replicating properties of the vectors. The recombinant vector is introduced into a host cell and multiplied in the host cell. Common vectors are plasmids, phagemids, and yeast artificial chromosomes. (Li Shengbin) [1]
Cloning vector
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- Chinese name
- Cloning vector
- Foreign name
- Cloning Vector
- Obtain
- Virus, plasmid or higher biological cell
- As
- DNA as a cloning vector
- Cloning vectors usually use DNA obtained from viruses, plasmids or higher biological cells as cloning vectors, and insert exogenous DNA fragments of appropriate size on the vectors, taking care not to destroy the self-replicating properties of the vectors. The recombinant vector is introduced into a host cell and multiplied in the host cell. Common vectors are plasmids, phagemids, and yeast artificial chromosomes. (Li Shengbin) [1]
- Requirements for the vector: Can reproduce and reproduce in the host cell, and preferably has a high autonomous replication ability.
- Easy to enter host cells, and the higher the entry efficiency, the better.
- It is easy to insert foreign nucleic acid fragments. After insertion, it does not affect its entry into host cells and its replication in cells. This requires a suitable restriction endonuclease site on the vector DNA.
- It is easy to isolate and purify from the host cell, which is convenient for recombination operation.
- There are markers that can be easily identified and screened. When they enter the host cell or carry foreign nucleic acid sequences into the host cell, they can be easily identified and isolated. This is between cloning