What Is a Helicase?

Helicases are a class of enzymes that release hydrogen bonds, not an enzyme. They are enzymes that break down DNA by hydrolyzing ATP to provide energy. They often rely on the existence of single strands and recognize the single-stranded structure of the replication fork. Generally plays a role in catalyzing the unwinding of double-stranded DNA or RNA during DNA or RNA replication.

Helicase

DNA helicase

Usually a fluid protein loop

There are two types of views

1. No: Helicase is required for DNA replication, but similar to DNA replication

1, single

1.E.coli and

Nucleic acid isothermal amplification technology and its application:

In vitro culture of pathogenic microorganisms has always been the "gold standard" for pathogen diagnosis. According to estimates by microbiologists, only about 1% of bacteria can be cultured using culture techniques. In the past century, nucleic acid-based detection technology, represented by polymerase chain reaction (PCR), has developed rapidly, providing the possibility for accurate detection and diagnosis of other pathogens. Needless to say, the PCR technology invented by Kary Mtlllis was a revolutionary breakthrough in the field of molecular biology in the 1980s. Perhaps he himself did not expect that PCR technology would play such an important role in the fields of molecular biology, medicine, and law. After decades of improvement, the PCR method has evolved from qualitative to quantitative, capable of amplifying billions of specific nucleic acid fragments from several copies or a single cell within a few hours, and the specificity has also been greatly Of improvement. However, since the birth of PCR technology, it has not been able to get rid of the limitations of relying on sophisticated instruments and equipment, making PCR-based nucleic acid amplification detection technology unable to be more widely promoted and applied.

Based on this strong demand, detection technology based on nucleic acid isothermal amplification technology has developed rapidly. Not only are isothermal technologies of various mechanisms born, but some technologies are quite mature, completing the transition from laboratory to practical applications, and are gradually being widely used in the fields of molecular biology, medicine, and law. Especially in the field of clinical and on-site (point-of-care) rapid diagnosis technology, nucleic acid isothermal amplification technology shows its outstanding superiority. More importantly, because isothermal nucleic acid amplification technology does not require a time course of temperature change and it has got rid of its dependence on sophisticated equipment, we can achieve rapid and high-throughput detection of pathogens.

Note

Transcriptases are a class of enzymes, RNA polymerases I, II, and III. Their respective roles are that RNA polymerase I is responsible for the transcription of rRNA genes, RNA polymerase II is responsible for the transcription of mRNA precursors, and RNA polymerase III is responsible for the generation of tRNA, 5SRNA, Alu sequences and other small RNAs.

Of course, transcription also includes helicase. Also mention: translation is mainly the large ribosomal subunits acting as peptidyltransferases.